Mouse/Rat SIGNR3/CD209d Antibody

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Detection of Mouse and Rat SIGNR3/CD209d by Western Blot.
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Mouse/Rat SIGNR3/CD209d Antibody Summary

Species Reactivity
Mouse, Rat
Detects mouse and rat SIGNR3/CD209d in Western blots. In Western blots, 100% cross-reactivity with recombinant mouse (rm) SIGNR1, 50% cross-reactivity with rmSIGNR7, and no cross-reactivity with rmSIGNR4 is observed. In direct ELISAs, no cross-reactivity with recombinant human (rh) DC-SIGN, rhDC-SIGNR, rmSIGNR1, rmSIGNR4, or rmSIGNR7 is observed.
Monoclonal Rat IgG2A Clone # 728804
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse SIGNR3/CD209d
Accession # Q91ZW8
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat SIGNR3/CD209d antibody by Western Blot. View Larger

Detection of Mouse and Rat SIGNR3/CD209d by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat SIGNR3/CD209d Monoclonal Antibody (Catalog # MAB7220) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for SIGNR3/CD209d at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIGNR3/CD209d

SIGNR3 (designated CD209d) is a 237 amino acid (aa), ~34 kDa type II transmembrane glycoprotein that is one of several mouse orthologs of human DC-SIGN (CD209). SIGNR3 is a calcium-binding C-type lectin that is expressed on myeloid mononuclear cells and a subpopulation of dendritic cells in the dermis, blood, and most lymphoid organs. Like human DC-SIGN, SIGNR3 recognizes high-mannose and fucose-terminated glycans and protects against pathogens such as Candida albicans and Mycobacterium tuberculosis. Within the region used as an immunogen, mouse and rat SIGNR3 share 91% aa sequence identity. A 207 aa isoform lacks aa 45-74, which includes the transmembrane domain.

Long Name
DC-SIGN-related Protein 3
Entrez Gene IDs
170779 (Mouse); 363856 (Rat)
Alternate Names

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