Mouse/Rat SIGNR3/CD209d Antibody

  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat SIGNR3/CD209d in Western blots. In Western blots, 100% cross-reactivity with recombinant mouse (rm) SIGNR1, 50% cross-reactivity with rmSIGNR7, and no cross-reactivity with rmSIGNR4 is observed. In direct ELISAs, no cross-reactivity with recombinant human (rh) DC-SIGN, rhDC-SIGNR, rmSIGNR1, rmSIGNR4, or rmSIGNR7 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 728804
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse SIGNR3/CD209d
    Glu82-Lys237
    Accession # Q91ZW8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat SIGNR3/CD209d by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat SIGNR3/CD209d Monoclonal Antibody (Catalog # MAB7220) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for SIGNR3/CD209d at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SIGNR3/CD209d
SIGNR3 (designated CD209d) is a 237 amino acid (aa), ~34 kDa type II transmembrane glycoprotein that is one of several mouse orthologs of human DC-SIGN (CD209). SIGNR3 is a calcium-binding C-type lectin that is expressed on myeloid mononuclear cells and a subpopulation of dendritic cells in the dermis, blood, and most lymphoid organs. Like human DC-SIGN, SIGNR3 recognizes high-mannose and fucose-terminated glycans and protects against pathogens such as Candida albicans and Mycobacterium tuberculosis. Within the region used as an immunogen, mouse and rat SIGNR3 share 91% aa sequence identity. A 207 aa isoform lacks aa 45-74, which includes the transmembrane domain.
  • Long Name:
    DC-SIGN-related Protein 3
  • Entrez Gene IDs:
    170779 (Mouse); 363856 (Rat)
  • Alternate Names:
    CD209d; SIGNR3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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