SMOC-1 (secreted, or SPARC-related, modular calcium-binding protein 1) is a 70‑90 kDa secreted glycoprotein that is a member of the SPARC family of matricellular molecules (1). Mature mouse SMOC-1 is 427 amino acids (aa) in length. It contains one Kazal-like domain (aa 42‑86), two thyroglobulin type-1 segments (aa 91‑157 and 223‑291) and two functional EF-hand sequences (aa 358‑393 and 395‑430) (1, 2). Two splice variants contain an insertion of eleven aa after Lys174, with one of these also including an alternate start site at Met65 (3). Mature mouse SMOC-1 shares 99% aa identity with rat SMOC-1 and 92% aa identity with human, canine and bovine SMOC-1. The principal difference between rodents and other mammals is an additional 19 aa near the C-terminus of rodent SMOC-1. Like other matricellular proteins, SMOC-1 is primarily expressed in basement membranes, although it has also been found in other extracellular matrices and the oocyte zona pellucida (1). It is present early in mouse embryogenesis, and is produced by cells deriving from all three germ layers (4). Recombinant bacterially produced human SMOC-1 and SMOC-2 were both shown to bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin (2). A signaling role for SMOC-1 was shown in rat mesangial cells: induction of nitric oxide in response to inflammatory cytokines downregulates SMOC-1 which, in turn, downregulates expression of TGF-beta and TGF-beta -regulated genes. This mechanism is proposed to limit the profibrotic effects of TGF-beta, for example in the glomerulus (5). In Xenopus, the SMOC paralog has been shown to antagonize BMP activity.
Mouse/Rat SMOC‑1 Alexa Fluor® 488‑conjugated Antibody
R&D Systems | Catalog # AF5550G
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
His26-Val452
Accession # AAD41590
Specificity
Clonality
Host
Isotype
Applications for Mouse/Rat SMOC‑1 Alexa Fluor® 488‑conjugated Antibody
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: SMOC-1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional SMOC-1 Products
Product Documents for Mouse/Rat SMOC‑1 Alexa Fluor® 488‑conjugated Antibody
Product Specific Notices for Mouse/Rat SMOC‑1 Alexa Fluor® 488‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
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