Mouse/Rat TREM-1 Quantikine ELISA Kit

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MTRM10
Mouse/Rat TREM-1 ELISA Standard Curve
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Product Details
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Citations (2)
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Mouse/Rat TREM-1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
5.4 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat TREM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat TREM-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse or rat TREM-1 in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse TREM-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural TREM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring TREM-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 26 26 26
Mean 79.8 248 1222 79.3 227 1160
Standard Deviation 4.5 22 51.6 6.3 14 62.2
CV% 5.6 8.9 4.2 7.9 6.2 5.4

Recovery

The recovery of TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=5) 98 87-109
Mouse EDTA Plasma (n=6) 99 90-108
Mouse Heparin Plasma (n=5) 101 89-110
Mouse Serum (n=5) 98 81-106
Mouse Tissue Homogenates (n=2) 100 91-104
Rat Cell Culture Supernates (n=5) 97 90-109
Rat EDTA Plasma (n=5) 100 85-112
Rat Heparin Plasma (n=5) 99 85-113
Rat Serum (n=4) 107 84-120
Rat Tissue Homogenates (n=2) 99 95-103

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TREM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat TREM-1 ELISA Linearity
Mouse/Rat TREM-1 ELISA Linearity

Data Examples

Mouse/Rat TREM-1 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TREM-1

The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.

Long Name:
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs:
54210 (Human); 58217 (Mouse)
Alternate Names:
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat TREM-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Targeted endothelial gene deletion of Triggering Receptor Expressed on Myeloid cells-1 protects mice during septic shock
    Authors: L Jolly, K Carrasco, M Derive, J Lemarié, A Boufenzer, S Gibot
    Cardiovasc. Res., 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
  2. TREM-1 promotes survival during septic shock in mice.
    Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
    Eur. J. Immunol., 2007;37(2):456-66.
    Species: Mouse
    Sample Types: Tissue Homogenates

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