< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Mouse/Rat WISP-1/CCN4 immunoassay is a 4.5 hour solid-phase ELISA designed to measure WISP-1 in mouse or rat cell culture supernates, tissue lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse WISP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse WISP-1. Results obtained using natural mouse or rat WISP-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural WISP-1.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to
assess inter-assay precision. Assays were
performed by at least three technicians using two lots of kit components
The recovery of WISP-1 spiked to levels throughout the range of the assay was evaluated.
Average % Recovery
Cell Culture Samples (n=4)
Cell Lysates (n=4)
EDTA Plasma (n=4)
Heparin Plasma (n=4)
To assess the linearity of the assay, samples containing high concentrations of WISP-1 were serially diluted with
Calibrator Diluent to produce samples with values within the dynamic
range of the assay.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
WISP-1 belongs to the CCN (CTGF/CYR61/NOV) family of secreted proteins that share a common multimodular organization. Each family member has an IGFBP domain, a von Willebrand type C domain, a thrombospondin 1 domain and a C-terminal cysteine knot domain. Depending on cell context, WISP-1 can either promote or inhibit tumorigenesis.
Wnt-1 Induced Secreted Protein 1
Entrez Gene IDs:
8840 (Human); 22402 (Mouse)
CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
50 µL Assay Diluent
Add 50 µL of Assay Diluent to each well.
50 µL Standard, Control, or Sample
Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
100 µL Conjugate
Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
Aspirate and wash 4 times.
100 µL Substrate Solution
Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
100 µL Stop Solution
Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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