< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Mouse/Rat WISP-1/CCN4 immunoassay is a 4.5 hour solid-phase ELISA designed to measure WISP-1 in mouse or rat cell culture supernates, tissue lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse WISP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse WISP-1. Results obtained using natural mouse or rat WISP-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural WISP-1.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to
assess inter-assay precision. Assays were
performed by at least three technicians using two lots of kit components.
The recovery of WISP-1 spiked to levels throughout the range of the assay was evaluated.
Average % Recovery
Cell Culture Samples (n=4)
Cell Lysates (n=4)
EDTA Plasma (n=4)
Heparin Plasma (n=4)
To assess the linearity of the assay, samples containing high concentrations of WISP-1 were serially diluted with
Calibrator Diluent to produce samples with values within the dynamic
range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
WISP-1 belongs to the CCN (CTGF/CYR61/NOV) family of secreted proteins that share a common multimodular organization. Each family member has an IGFBP domain, a von Willebrand type C domain, a thrombospondin 1 domain and a C-terminal cysteine knot domain. Depending on cell context, WISP-1 can either promote or inhibit tumorigenesis.
Wnt-1 Induced Secreted Protein 1
Entrez Gene IDs
8840 (Human); 22402 (Mouse);
CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1;
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