Mouse/Rat WISP-1/CCN4 Quantikine ELISA Kit

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MWSP10
Mouse/Rat WISP-1/CCN4 ELISA Standard Curve
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Product Details
Procedure
Citations (1)
FAQs
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Reviews (1)

Mouse/Rat WISP-1/CCN4 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL), Urine (50 uL)
Sensitivity
2.74 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse and rat WISP-1/CCN4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat WISP-1/CCN4 immunoassay is a 4.5 hour solid-phase ELISA designed to measure WISP-1 in mouse or rat cell culture supernates, tissue lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse WISP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate
the recombinant mouse WISP-1. Results obtained using natural mouse or rat WISP-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural WISP-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 33.8 106 323 37.3 93.7 288
Standard Deviation 1.58 4.06 15.9 3.59 6.16 11.1
CV% 4.7 3.8 4.9 9.6 6.6 3.9

Recovery

The recovery of WISP-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 104 89-119
Cell Lysates (n=4) 89 80-111
EDTA Plasma (n=4) 110 96-119
Heparin Plasma (n=4) 104 81-120
Serum (n=4) 104 90-119
Urine (n=4) 107 90-120

Linearity

To assess the linearity of the assay, samples containing high concentrations of WISP-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat WISP-1/CCN4 ELISA Linearity

Data Examples

Mouse/Rat WISP-1/CCN4 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: WISP-1/CCN4

WISP-1 belongs to the CCN (CTGF/CYR61/NOV) family of secreted proteins that share a common multimodular organization. Each family member has an IGFBP domain, a von Willebrand type C domain, a thrombospondin 1 domain and a C-terminal cysteine knot domain. Depending on cell context, WISP-1 can either promote or inhibit tumorigenesis.

Long Name:
Wnt-1 Induced Secreted Protein 1
Entrez Gene IDs:
8840 (Human); 22402 (Mouse)
Alternate Names:
CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citation for Mouse/Rat WISP-1/CCN4 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes
    Authors: M Rödiger, MW Werno, I Wilhelmi, C Baumeier, D Hesse, N Wettschure, S Offermanns, K Song, M Krau beta, A Schürmann
    Mol Metab, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates

FAQs

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Mouse/Rat WISP-1/CCN4 Quantikine ELISA Kit
By Anonymous on 07/12/2017
Sample Tested: Cell Culture Media