Detection of Siglec‑H in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse Siglec‑H PE‑conjugated Monoclonal Antibody (Catalog # FAB7319P) or (B) Rat IgG1 Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Siglec-H, a murine CD33-related Siglec-like molecule and a member of the Sialic Acid-binding Immunoglobulin (Ig)-like Lectin (Siglec) family, is expressed specifically on Plasmacytoid Dendritic Cell (pDC) precursors in the bone marrow, spleen, blood, and lymph nodes.
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