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Mouse Siglec-H PE-conjugated Antibody

Catalog #: FAB7319P Datasheet / COA / SDS

Discontinued Product

FAB7319P has been discontinued.
View all Siglec-H products.
Detection of Siglec‑H in Mouse Splenocytes by Flow Cytometry.
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Mouse Siglec-H PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Siglec-H in flow cytometry.
Source
Monoclonal Rat IgG1 Clone # 730407
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Siglec-H
Met1-Gly269
Accession # NP_848821
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Siglec-H antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Siglec‑H in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse Siglec-H PE-conjugated Monoclonal Antibody (Catalog # FAB7319P) or (B) Rat IgG1Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Siglec-H

Siglec-H, a murine CD33-related Siglec-like molecule and a member of the Sialic Acid-binding Immunoglobulin (Ig)-like Lectin (Siglec) family, is expressed specifically on Plasmacytoid Dendritic Cell (pDC) precursors in the bone marrow, spleen, blood, and lymph nodes.

Long Name
Sialic Acid Binding Ig-like Lectin 7
Entrez Gene IDs
233274 (Mouse)
Alternate Names
6430529G09Rik; Siglech sialic acid binding Ig-like lectin H; SiglecH; Siglec-H

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