Mouse SIGNR3/CD209d Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: SIGNR3/CD209d

SIGNR3 (Specific Intercellular adhesion molecule-3-Grabbing Non-integrin Related gene 3; also CD209d and DC-SIGNR3) is a 30-34 kDa member of the C-type lectin family of proteins. It is found on macrophages, DC and monocytes, and appears to bind both high mannose and fucose moieties on the surface of extracellular pathogens. It is particularly important in clearing Mycobacterium tuberculosis. Upon ligation, SIGNR3 is internalized and presumably enters the endosomal system. Notably, and unlike in human, there are multiple SIGNs in mouse, and SIGNR3 most closely resembles the biology of human DC-SIGN. Mouse SIGNR3 is a 237 amino acid (aa) type II transmembrane glycoprotein. It contains a 54 aa N-terminal cytoplasmic region plus a 162 aa C-terminal extracellular domain (aa 76-237) that possesses a C-type lectin domain (aa 106-228). It is suggested that mouse SIGNR3 may oligomerize. There are two potential splice variants. One possesses an alternative start site 23 aa upstream of the standard site, while a second shows a deletion of aa 45-74. Over aa 82-237, mouse SIGNR3 shares 89% and 59% aa sequence identity with rat and human SIGNR3, respectively.

Product Datasheets

Related Research Areas

• C-type Lectin Family
• C-type Lectin Receptors
• Dendritic Cell Adhesion and Migration
• Dendritic Cell Pathogen Recognition/Uptake