Mouse TLR12 Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # FAB8086AFP405

R&D Systems
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1229C
View all TLR12 Primary Antibodies »

Product Specifications

Specificity

Detects mouse TLR12 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Mouse TLR12 Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TLR12

Toll-like receptors (TLRs) are type I transmembrane proteins that activate the immune system in response to molecular patterns derived from microbial pathogens. Some TLRs are expressed on the cell surface (TLR1, 2, 4, 5, 6, 10), while others are found in endosomal structures (TLR3, 7, 8, 9, 11, 12, 13). TLRs contain a large number of leucine-rich repeats (LRRs) in their ectodomains and a cytoplasmic tail with one signal transducing Toll/IL-1 receptor (TIR) domain (1, 2). Mature mouse TLR12 is an approximately 100 kDa glycoprotein that consists of a 688 amino acid (aa) ectodomain with 17 LRRs, a 21 aa transmembrane segment, and a 176 aa cytoplasmic region (3). Within the ectodomain, mouse TLR12 shares 80% aa sequence identity with rat TLR12. TLR12 is expressed in dendritic cells, macrophages, and neurons, and in the uterus, liver, kidney, and bladder (3-6). It is up-regulated in the adipose tissue of obesity-prone ob/ob mice fed a high fat diet (7). The intracellular trafficking and sorting of several TLRs (including TLR12) is dependent on the endoplasmic reticulum resident protein UNC93B1 (8, 9). TLR12 can associate into homodimers as well as heterodimers with TLR11 (4). TLR11 can additionally heterodimerize with TLR3 and TLR7 (4, 8). TLR12 is required for the inflammatory response against uropathogenic bacteria and Toxoplasma gondii (3, 4). It directly binds Profilin from T. gondii, and it cooperates with TLR11 in protecting against T. gondii infection in vivo (4).

References

  1. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  2. Song, D.H. and J.O. Lee (2012) Immunol. Rev. 250:216.
  3. Zhang, D. et al. (2004) Science 303:1522.
  4. Koblansky, A.A. et al. (2013) Immunity 38:119.
  5. Mishra, B.B. et al. (2008) J. Neuroinflam. 5:53.
  6. Hickey, D.K. et al. (2013) Innate Immun. 19:121.
  7. Kim, S.J. et al. (2012) J. Nutr. Biochem. 23:113.
  8. Andrade, W.A. et al. (2013) Cell Host Microbe 13:42.
  9. Lee, B.L. et al. (2013) eLife 2:e00291.

Long Name

Toll-like Receptor 12

Alternate Names

Gm1365

Entrez Gene IDs

384059 (Mouse)

Gene Symbol

TLR12

UniProt

Q6QNU9

Additional TLR12 Products

Product Documents for Mouse TLR12 Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse TLR12 Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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