Mouse TLR12 Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TLR12 in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1229C
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse TLR12
    Thr22-Lys709
    Accession # Q6QNU9
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse TLR12 by Western Blot. Western blot shows lysates of either HEK293T human embryonic kidney cell line mock transfected or HEK293T human embryonic kidney cell line transfected with mouse TLR12. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Mouse TLR12 Monoclonal Antibody (Catalog # MAB8086) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TLR12 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TLR12 in HEK293 Human Cell Line Transfected with Mouse TLR12 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse TLR12 and eGFP was stained with and either (A) Rabbit Anti-Mouse TLR12 Monoclonal Antibody (Catalog # MAB8086) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: TLR12
Toll-like receptors (TLRs) are type I transmembrane proteins that activate the immune system in response to molecular patterns derived from microbial pathogens. Some TLRs are expressed on the cell surface (TLR1, 2, 4, 5, 6, 10), while others are found in endosomal structures (TLR3, 7, 8, 9, 11, 12, 13). TLRs contain a large number of leucine-rich repeats (LRRs) in their ectodomains and a cytoplasmic tail with one signal transducing Toll/IL-1 receptor (TIR) domain (1, 2). Mature mouse TLR12 is an approximately 100 kDa glycoprotein that consists of a 688 amino acid (aa) ectodomain with 17 LRRs, a 21 aa transmembrane segment, and a 176 aa cytoplasmic region (3). Within the ectodomain, mouse TLR12 shares 80% aa sequence identity with rat TLR12. TLR12 is expressed in dendritic cells, macrophages, and neurons, and in the uterus, liver, kidney, and bladder (3-6). It is up-regulated in the adipose tissue of obesity-prone ob/ob mice fed a high fat diet (7). The intracellular trafficking and sorting of several TLRs (including TLR12) is dependent on the endoplasmic reticulum resident protein UNC93B1 (8, 9). TLR12 can associate into homodimers as well as heterodimers with TLR11 (4). TLR11 can additionally heterodimerize with TLR3 and TLR7 (4, 8). TLR12 is required for the inflammatory response against uropathogenic bacteria and Toxoplasma gondii (3, 4). It directly binds Profilin from T. gondii, and it cooperates with TLR11 in protecting against T. gondii infection in vivo (4).
  • References:
    1. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
    2. Song, D.H. and J.O. Lee (2012) Immunol. Rev. 250:216.
    3. Zhang, D. et al. (2004) Science 303:1522.
    4. Koblansky, A.A. et al. (2013) Immunity 38:119.
    5. Mishra, B.B. et al. (2008) J. Neuroinflam. 5:53.
    6. Hickey, D.K. et al. (2013) Innate Immun. 19:121.
    7. Kim, S.J. et al. (2012) J. Nutr. Biochem. 23:113.
    8. Andrade, W.A. et al. (2013) Cell Host Microbe 13:42.
    9. Lee, B.L. et al. (2013) eLife 2:e00291.
  • Long Name:
    Toll-like Receptor 12
  • Entrez Gene IDs:
    384059 (Mouse)
  • Alternate Names:
    Gm1365; TLR12; toll-like receptor 12
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 42  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG Antibody

WB AF008 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse TLR12 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information