Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1229C
View all TLR12 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse TLR12
Thr22-Lys709
Accession # Q6QNU9

Specificity

Detects mouse TLR12 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse TLR12 Antibody

Detection of Mouse TLR12 antibody by Western Blot.

Detection of Mouse TLR12 by Western Blot.

Western blot shows lysates of either HEK293T human embryonic kidney cell line mock transfected or HEK293T human embryonic kidney cell line transfected with mouse TLR12. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Mouse TLR12 Monoclonal Antibody (Catalog # MAB8086) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TLR12 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TLR12 antibody in HEK293 Human Cell Line Transfected with Mouse TLR12 and eGFP antibody by Flow Cytometry.

Detection of TLR12 in HEK293 Human Cell Line Transfected with Mouse TLR12 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse TLR12 and eGFP was stained with and either (A) Rabbit Anti-Mouse TLR12 Monoclonal Antibody (Catalog # MAB8086) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Applications for Mouse TLR12 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse TLR12 and eGFP

Western Blot

0.5 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with mouse TLR12

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TLR12

Toll-like receptors (TLRs) are type I transmembrane proteins that activate the immune system in response to molecular patterns derived from microbial pathogens. Some TLRs are expressed on the cell surface (TLR1, 2, 4, 5, 6, 10), while others are found in endosomal structures (TLR3, 7, 8, 9, 11, 12, 13). TLRs contain a large number of leucine-rich repeats (LRRs) in their ectodomains and a cytoplasmic tail with one signal transducing Toll/IL-1 receptor (TIR) domain (1, 2). Mature mouse TLR12 is an approximately 100 kDa glycoprotein that consists of a 688 amino acid (aa) ectodomain with 17 LRRs, a 21 aa transmembrane segment, and a 176 aa cytoplasmic region (3). Within the ectodomain, mouse TLR12 shares 80% aa sequence identity with rat TLR12. TLR12 is expressed in dendritic cells, macrophages, and neurons, and in the uterus, liver, kidney, and bladder (3-6). It is up-regulated in the adipose tissue of obesity-prone ob/ob mice fed a high fat diet (7). The intracellular trafficking and sorting of several TLRs (including TLR12) is dependent on the endoplasmic reticulum resident protein UNC93B1 (8, 9). TLR12 can associate into homodimers as well as heterodimers with TLR11 (4). TLR11 can additionally heterodimerize with TLR3 and TLR7 (4, 8). TLR12 is required for the inflammatory response against uropathogenic bacteria and Toxoplasma gondii (3, 4). It directly binds Profilin from T. gondii, and it cooperates with TLR11 in protecting against T. gondii infection in vivo (4).

References

  1. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  2. Song, D.H. and J.O. Lee (2012) Immunol. Rev. 250:216.
  3. Zhang, D. et al. (2004) Science 303:1522.
  4. Koblansky, A.A. et al. (2013) Immunity 38:119.
  5. Mishra, B.B. et al. (2008) J. Neuroinflam. 5:53.
  6. Hickey, D.K. et al. (2013) Innate Immun. 19:121.
  7. Kim, S.J. et al. (2012) J. Nutr. Biochem. 23:113.
  8. Andrade, W.A. et al. (2013) Cell Host Microbe 13:42.
  9. Lee, B.L. et al. (2013) eLife 2:e00291.

Long Name

Toll-like Receptor 12

Alternate Names

Gm1365

Entrez Gene IDs

384059 (Mouse)

Gene Symbol

TLR12

UniProt

Q6QNU9

Additional TLR12 Products

Product Documents for Mouse TLR12 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse TLR12 Antibody

For research use only

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