Mouse TLR12 Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC8086R-025
Detection  of TLR12 in HEK293 Human Cell Line Transfected with Mouse TLR12 and eGFP by  Flow Cytometry.
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Mouse TLR12 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TLR12 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1229C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse TLR12
Thr22-Lys709
Accession # Q6QNU9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of TLR12 in HEK293 Human Cell Line Transfected with Mouse TLR12 and eGFP by  Flow Cytometry. View Larger

Detection of TLR12 in HEK293 Human Cell Line Transfected with Mouse TLR12 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse TLR12 and eGFP was stained with  and either (A) Rabbit Anti-Mouse TLR12 Alexa Fluor® 647‑conjugated Monoclonal Antibody
(Catalog # IC8086R) or (B) Normal Rabbit IgG Alexa Fluor 647 Control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR12

Toll-like receptors (TLRs) are type I transmembrane proteins that activate the immune system in response to molecular patterns derived from microbial pathogens. Some TLRs are expressed on the cell surface (TLR1, 2, 4, 5, 6, 10), while others are found in endosomal structures (TLR3, 7, 8, 9, 11, 12, 13). TLRs contain a large number of leucine-rich repeats (LRRs) in their ectodomains and a cytoplasmic tail with one signal transducing Toll/IL-1 receptor (TIR) domain (1, 2). Mature mouse TLR12 is an approximately 100 kDa glycoprotein that belongs to the TLR11 family of TLRs. It consists of a 688 amino acid (aa) ectodomain with 17 LRRs, a 21 aa transmembrane segment, and a 176 aa cytoplasmic region (3). Over aa21-709, mouse TLR12 shares 80% aa sequence identity with rat TLR12. There is no human counterpart. TLR12 is expressed in dendritic cells, macrophages, and neurons, and in the uterus, liver, kidney, and bladder (3-6). It is up-regulated in the adipose tissue of obesity-prone ob/ob mice fed a high fat diet (7). The intracellular trafficking and sorting of several TLRs (including TLR12) is dependent on the endoplasmic reticulum resident protein UNC93B1 (8, 9). TLR12 can associate into homodimers as well as heterodimers with TLR11 (4). TLR11 can additionally heterodimerize with TLR3 and TLR7 (4, 8). TLR12 is required for the inflammatory response against uropathogenic bacteria and Toxoplasma gondii (3, 4). It directly binds Profilin from T. gondii, and it cooperates with TLR11 in protecting against T. gondii infection in vivo (4).

References
  1. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  2. Song, D.H. and J.O. Lee (2012) Immunol. Rev. 250:216.
  3. Zhang, D. et al. (2004) Science 303:1522.
  4. Koblansky, A.A. et al. (2013) Immunity 38:119.
  5. Mishra, B.B. et al. (2008) J. Neuroinflammation 5:53.
  6. Hickey, D.K. et al. (2013) Innate Immun. 19:121.
  7. Kim, S.J. et al. (2012) J. Nutr. Biochem. 23:113.
  8. Andrade, W.A. et al. (2013) Cell Host Microbe 13:42.
  9. Lee, B.L. et al. (2013) ELife 2:e00291.
Long Name
Toll-like Receptor 12
Entrez Gene IDs
384059 (Mouse)
Alternate Names
Gm1365; TLR12; toll-like receptor 12

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