Mouse uPAR PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB531P-100UG
Detection of uPAR in Mouse Blood Monocytes by Flow Cytometry.
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Citations (2)
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Mouse uPAR PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse uPAR in Western blots. In Western blots, this antibody does not cross-react with recombinant human uPAR.
Source
Monoclonal Rat IgG2A Clone # 109801
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse uPAR isoform 1
Leu24-Thr297
Accession # P35456
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of uPAR antibody in Mouse Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of uPAR in Mouse Blood Monocytes by Flow Cytometry. Mouse whole blood monocytes were stained with Rat Anti-Mouse uPAR PE-conjugated Monoclonal Antibody (Catalog # FAB531P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of uPAR in Raw264.7 Mouse cell line by Flow Cytometry. Raw264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse uPAR PE-conjugated Monoclonal Antibody (Catalog # FAB531P, filled histogram) or isotype control antibody (IC006P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: uPAR

The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is highly species-specific. Human uPA binds rmuPAR at a lower affinity compared to rhuPAR.

References
  1. Dear, A.E. and R.L. Medcalf (1998) Eur. J. Biochemistry 252:185.
Long Name
Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs
5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)
Alternate Names
CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

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Citations for Mouse uPAR PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Senolytic CAR T cells reverse senescence-associated pathologies
    Authors: C Amor, J Feucht, J Leibold, YJ Ho, C Zhu, D Alonso-Cur, J Mansilla-S, JA Boyer, X Li, T Giavridis, A Kulick, S Houlihan, E Peerschke, SL Friedman, V Ponomarev, A Piersigill, M Sadelain, SW Lowe
    Nature, 2020-06-17;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Bone marrow-derived immature myeloid cells are a main source of circulating suPAR contributing to proteinuric kidney disease
    Nat. Med, 2016-12-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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