MRPL12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-95243

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MRPL12 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human MRPL12 (NP_002940.2). MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MRPL12 Antibody - BSA Free

MRPL12 Antibody - BSA Free

Western Blot: MRPL12 Antibody - BSA Free [MRPL12] -

Western Blot: MRPL12 Antibody - BSA Free [MRPL12] - Western blot analysis of various lysates using MRPL12 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 ug per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
MRPL12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody - BSA Free [MRPL12] -

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody - BSA Free [MRPL12] - Immunofluorescence analysis of U-2 OS cells using MRPL12 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
MRPL12 Antibody - BSA Free

Immunohistochemistry: MRPL12 Antibody - BSA Free [MRPL12] -

Immunohistochemistry: MRPL12 Antibody - BSA Free [MRPL12] - Immunohistochemistry analysis of MRPL12 in paraffin-embedded human thyroid cancer tissue using MRPL12 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
MRPL12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody - BSA Free [MRPL12] -

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody - BSA Free [MRPL12] - Immunofluorescence analysis of L-929 cells using MRPL12 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
MRPL12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody - BSA Free [MRPL12] -

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody - BSA Free [MRPL12] - Immunofluorescence analysis of C6 cells using MRPL12 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for MRPL12 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MRPL12

MRPL12, also known as 39 S ribosomal protein L12, mitochondrial, is a 21.3 kDa, 198 amino acid protein that is involved in mitochondrial protein synthesis by forming homodimers in 39S subunits. Current research is being conducted to identify the effect of the protein on diseases such as prostate cancer and prostatitis. The protein interacts in translation pathways with other proteins such as ICT1, MRPL3, SRRM2, PPP2R2B, and TMEM51.

Alternate Names

5c5-2, FLJ60124, L12mt, MGC8610, mitochondrial ribosomal protein L12, MRP-L12, MRP-L31/34, MRPL7, MRPL7/L12, RPML1239S ribosomal protein L12, mitochondrial

Gene Symbol

MRPL12

Additional MRPL12 Products

Product Documents for MRPL12 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MRPL12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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