MRPL28 Antibody (3B9F2)

Novus Biologicals | Catalog # NBP3-15267

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3B9F2 expressed in HEK293
View all MRPL28 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MRPL28 (NP_006419.2). MPLHKYPVWLWKRLQLREGICSRLPGHYLRSLEEERTPTPVHYRPHGAKFKINPKNGQRERVEDVPIPIYFPPESQRGLWGGEGWILGQIYANNDKLSKR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MRPL28 Antibody (3B9F2)

Western Blot: MRPL28 Antibody (3B9F2) [NBP3-15267]

Western Blot: MRPL28 Antibody (3B9F2) [NBP3-15267]

Western Blot: MRPL28 Antibody (3B9F2) [NBP3-15267] - Analysis of extracts of various cell lines, using MRPL28 antibody (NBP3-15267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: MRPL28 Antibody (3B9F2) [NBP3-15267]

Immunocytochemistry/ Immunofluorescence: MRPL28 Antibody (3B9F2) [NBP3-15267]

Immunocytochemistry/Immunofluorescence: MRPL28 Antibody (3B9F2) [NBP3-15267] - Immunofluorescence analysis of PC-12 cells using MRPL28 Rabbit mAb (NBP3-15267) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: MRPL28 Antibody (3B9F2) [NBP3-15267]

Immunocytochemistry/ Immunofluorescence: MRPL28 Antibody (3B9F2) [NBP3-15267]

Immunocytochemistry/Immunofluorescence: MRPL28 Antibody (3B9F2) [NBP3-15267] - Immunofluorescence analysis of NIH/3T3 cells using MRPL28 Rabbit mAb (NBP3-15267) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation: MRPL28 Antibody (3B9F2) [NBP3-15267]

Immunoprecipitation: MRPL28 Antibody (3B9F2) [NBP3-15267]

Immunoprecipitation: MRPL28 Antibody (3B9F2) [NBP3-15267] - Analysis of 300ug extracts of K-562 cells using 3ug MRPL28 antibody (NBP3-15267). Western blot was performed from the immunoprecipitate using MRPL28 antibody (NBP3-15267) at a dilition of 1:1000.
MRPL28 Antibody (3B9F2)

Western Blot: MRPL28 Antibody (3B9F2) [MRPL28] -

Western Blot: MRPL28 Antibody (3B9F2) [MRPL28] - Western blot analysis of various lysates using MRPL28 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for MRPL28 Antibody (3B9F2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MRPL28

MRPL28, also known as 39 S ribosomal protein L28, mitochondrial, is a 30.2 kDa, 256 amino acid protein that is utilized in mitochondrial ribosomes as a member of the ribosomal protein L28P family. Diseases and disorders such as hepatitis, leukemia, bronchitis, inflammatory bowel disease, melanoma, vaccinia, influenza, esophagitis, hepatocellular carcinoma, Hodgkin's lymphoma, and rheumatoid arthritis are beings studied with this protein. The protein interacts with ENSG00000258947, TUBB4A, OXA1L, ICT1, and TUBB3.

Alternate Names

L28mt, MAAT139S ribosomal protein L28, mitochondrial, Melanoma antigen p15, melanoma-associated antigen recognised by cytotoxic T lymphocytes, Melanoma-associated antigen recognized by T lymphocytes, MGC8499, mitochondrial ribosomal protein L28, MRP-L28, p15

Gene Symbol

MRPL28

Additional MRPL28 Products

Product Documents for MRPL28 Antibody (3B9F2)

Certificate of Analysis

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Product Specific Notices for MRPL28 Antibody (3B9F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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