MSH6 Antibody (3E1)

Novus Biologicals | Catalog # NBP2-37537

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3E1
View all MSH6 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human MSH6 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MSH6 Antibody (3E1)

Western Blot: MSH6 Antibody (3E1)BSA Free [NBP2-37537]

Western Blot: MSH6 Antibody (3E1)BSA Free [NBP2-37537]

Western Blot: MSH6 Antibody (3E1) [NBP2-37537] - Western blot analysis using MSH6 mouse mAb against HEK293 (1), HCT116 (2), A549 (3), A431 (4), MCF-7 (5) and HepG2 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (3E1) - BSA Free [NBP2-37537]

Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (3E1) - BSA Free [NBP2-37537]

Immunocytochemistry/Immunofluorescence: MSH6 Antibody (3E1) [NBP2-37537] - Immunofluorescence analysis of Hela cells using MSH6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) - BSA Free [NBP2-37537]

Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) - BSA Free [NBP2-37537]

Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) [NBP2-37537] - Analysis of colon cancer tissues using MSH6 mouse mAb with DAB staining.
Flow Cytometry: MSH6 Antibody (3E1) - BSA Free [NBP2-37537]

Flow Cytometry: MSH6 Antibody (3E1) - BSA Free [NBP2-37537]

Flow Cytometry: MSH6 Antibody (3E1) [NBP2-37537] - Flow cytometric analysis of MCF-7 cells using MSH6 mouse mAb (green) and negative control (red).
Western Blot: MSH6 Antibody (3E1)BSA Free [NBP2-37537]

Western Blot: MSH6 Antibody (3E1)BSA Free [NBP2-37537]

Western Blot: MSH6 Antibody (3E1) [NBP2-37537] - Western blot analysis using MSH6 mAb against human MSH6 (AA: 217-395) recombinant protein. (Expected MW is 45.5 kDa)
Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) - BSA Free [NBP2-37537]

Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) - BSA Free [NBP2-37537]

Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) [NBP2-37537] - Analysis of rectum cancer tissues using MSH6 mouse mAb with DAB staining.
ELISA: MSH6 Antibody (3E1) - BSA Free [NBP2-37537]

ELISA: MSH6 Antibody (3E1) - BSA Free [NBP2-37537]

ELISA: MSH6 Antibody (3E1) [NBP2-37537] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for MSH6 Antibody (3E1)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSH6

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Alternate Names

DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein

Gene Symbol

MSH6

Additional MSH6 Products

Product Documents for MSH6 Antibody (3E1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MSH6 Antibody (3E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MSH6 Antibody (3E1)

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  • Q: I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.

    A: Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.

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