MSH6 Antibody (CL13844) - BSA Free

Novus Biologicals | Catalog # NBP3-24582

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL13844

Format

BSA Free
View all MSH6 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P52701, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MSH6 Antibody (CL13844) - BSA Free

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human colon shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Western Blot: MSH6 Antibody (CL13844) [NBP3-24582]

Western blot analysis in human cell line RH-30.

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Analysis in human testis and skeletal muscle tissues using NBP3-24582 antibody. Corresponding MSH6 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human testis cancer (embryonal carcinoma) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: MSH6 Antibody (CL13844) [NBP3-24582]

Staining of human lymph node shows strong nuclear positivity in germinal center cells.

Applications for MSH6 Antibody (CL13844) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1ug/ml
Application Notes
IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSH6

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Alternate Names

DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein

Gene Symbol

MSH6

Additional MSH6 Products

Product Documents for MSH6 Antibody (CL13844) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MSH6 Antibody (CL13844) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MSH6 Antibody (CL13844) - BSA Free

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  • Q: I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.

    A: Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.

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