MSH6 Antibody (CL13844) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43857
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL13844
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of P52701, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for MSH6 Antibody (CL13844) - Azide and BSA Free
![MSH6 Antibody (CL13844) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]](https://resources.rndsystems.com/images/products/nbp3-43857_mouse-msh6-mab-cl13844-azide-and-bsa-free-282202512111824.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]")
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]
Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.![MSH6 Antibody (CL13844) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]](https://resources.rndsystems.com/images/products/nbp3-43857_mouse-msh6-mab-cl13844-azide-and-bsa-free-282202512163651.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]")
![MSH6 Antibody (CL13844) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]](https://resources.rndsystems.com/images/products/nbp3-43857_mouse-msh6-mab-cl13844-azide-and-bsa-free-282202512321045.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]")
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]
Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.![MSH6 Antibody (CL13844) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]](https://resources.rndsystems.com/images/products/nbp3-43857_mouse-msh6-mab-cl13844-azide-and-bsa-free-28220251235169.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]")
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]
Staining of human colon shows strong nuclear positivity in glandular cells.![MSH6 Antibody (CL13844) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]](https://resources.rndsystems.com/images/products/nbp3-43857_mouse-msh6-mab-cl13844-azide-and-bsa-free-282202513112840.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]")
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]
Staining of human skeletal muscle shows no positivity in myocytes as expected.![MSH6 Antibody (CL13844) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]](https://resources.rndsystems.com/images/products/nbp3-43857_mouse-msh6-mab-cl13844-azide-and-bsa-free-28220251375650.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]")
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]
Staining of human lymph node shows strong nuclear positivity in germinal center cells.![MSH6 Antibody (CL13844) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]](https://resources.rndsystems.com/images/products/nbp3-43857_mouse-msh6-mab-cl13844-azide-and-bsa-free-28220251375668.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]")
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]
Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.![MSH6 Antibody (CL13844) - Azide and BSA Free Western Blot: MSH6 Antibody [NBP3-43857]](https://resources.rndsystems.com/images/products/nbp3-43857_mouse-msh6-mab-cl13844-azide-and-bsa-free-28220251337855.jpg "Western Blot: MSH6 Antibody [NBP3-43857]")
Western Blot: MSH6 Antibody [NBP3-43857]
Analysis in human cell line RH-30.![MSH6 Antibody (CL13844) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]](https://resources.rndsystems.com/images/products/nbp3-43857_mouse-msh6-mab-cl13844-azide-and-bsa-free-282202513355874.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]")
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]
Staining of human testis cancer (embryonal carcinoma) shows strong nuclear positivity in tumor cells.![MSH6 Antibody (CL13844) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]](https://resources.rndsystems.com/images/products/nbp3-43857_mouse-msh6-mab-cl13844-azide-and-bsa-free-282202513254767.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]")
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP3-43857]
Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.Applications for MSH6 Antibody (CL13844) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: MSH6
Alternate Names
DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein
Gene Symbol
MSH6
Additional MSH6 Products
Product Documents for MSH6 Antibody (CL13844) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MSH6 Antibody (CL13844) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for MSH6 Antibody (CL13844) - Azide and BSA Free
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Q: I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.
A: Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.
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