MSH6 Antibody (MSH6/3086)
Novus Biologicals | Catalog # NBP2-79712
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # MSH6/3086
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Product Specifications
Immunogen
Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary) (Uniprot: P52701)
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
163 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79846)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MSH6 Antibody (MSH6/3086)
![Western Blot: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.rndsystems.com/images/products/MSH6-Antibody-MSH6-3086-Western-Blot-NBP2-79712-img0005.jpg "Western Blot: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
Western Blot: MSH6 Antibody (MSH6/3086) [NBP2-79712]
Western Blot: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Western Blot Analysis of HCT116 cell lysate using MSH6 Antibody (MSH6/3086).![Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.rndsystems.com/images/products/MSH6-Antibody-MSH6-3086-Immunocytochemistry-Immunofluorescence-NBP2-79712-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
Immunocytochemistry/ Immunofluorescence: MSH6 Antibody (MSH6/3086) [NBP2-79712]
Immunocytochemistry/Immunofluorescence: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Immunofluorescence staining of PFA-fixed MCF-7 cells with MSH6 Antibody (MSH6/3086) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with RedDot (Red).![Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.rndsystems.com/images/products/MSH6-Antibody-MSH6-3086-Immunohistochemistry-Paraffin-NBP2-79712-img0003.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]
Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH6 Antibody (MSH6/3086).![Flow Cytometry: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.rndsystems.com/images/products/MSH6-Antibody-MSH6-3086-Flow-Cytometry-NBP2-79712-img0006.jpg "Flow Cytometry: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
Flow Cytometry: MSH6 Antibody (MSH6/3086) [NBP2-79712]
Flow Cytometry: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using MSH6 Mouse Monoclonal Antibody (MSH6/3086) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).![Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.rndsystems.com/images/products/MSH6-Antibody-MSH6-3086-Immunohistochemistry-Paraffin-NBP2-79712-img0002.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712]
Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Formalin-fixed, paraffin-embedded human Small Intestine stained with MSH6 Antibody (MSH6/3086).![Protein Array: MSH6 Antibody (MSH6/3086) [NBP2-79712]](https://resources.rndsystems.com/images/products/MSH6-Antibody-MSH6-3086-Protein-Array-NBP2-79712-img0004.jpg "Protein Array: MSH6 Antibody (MSH6/3086) [NBP2-79712]")
Protein Array: MSH6 Antibody (MSH6/3086) [NBP2-79712]
Protein Array: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Analysis of Protein Array containing >19,000 full-length human proteins using MSH6 Antibody (MSH6/3086).Applications for MSH6 Antibody (MSH6/3086)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: MSH6
Alternate Names
DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein
Gene Symbol
MSH6
UniProt
Additional MSH6 Products
Product Documents for MSH6 Antibody (MSH6/3086)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MSH6 Antibody (MSH6/3086)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for MSH6 Antibody (MSH6/3086)
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Q: I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.
A: Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.
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