![Knockout Validated: MSH6 Antibody [NBP1-33151]](https://resources.rndsystems.com/images/products/MSH6-Antibody-Western-Blot-NBP1-33151-img0008.jpg "Western Blot: MSH6 Antibody [NBP1-33151]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Zebrafish
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human MSH6. The exact sequence is proprietary.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MSH6 Antibody - BSA Free
Western Blot: MSH6 Antibody [NBP1-33151]
Western Blot: MSH6 Antibody [NBP1-33151] - Wild-type (WT) and MSH6 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH6 antibody [C1C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Western Blot: MSH6 Antibody [NBP1-33151]](https://resources.rndsystems.com/images/products/MSH6-Antibody-Western-Blot-NBP1-33151-img0007.jpg "Western Blot: MSH6 Antibody [NBP1-33151]")
Western Blot: MSH6 Antibody [NBP1-33151]
Western Blot: MSH6 Antibody [NBP1-33151] - Sample (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE; antibody diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: MSH6 Antibody [NBP1-33151]](https://resources.rndsystems.com/images/products/MSH6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33151-img0004.jpg "Immunocytochemistry/ Immunofluorescence: MSH6 Antibody [NBP1-33151]")
Immunocytochemistry/ Immunofluorescence: MSH6 Antibody [NBP1-33151]
Immunocytochemistry/Immunofluorescence: MSH6 Antibody [NBP1-33151] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.![Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-33151]](https://resources.rndsystems.com/images/products/MSH6-Antibody-Immunohistochemistry-Paraffin-NBP1-33151-img0005.jpg "Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-33151]")
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-33151]
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-33151] - Paraffin-embedded NCIN87 Xenograft, using antibody at 1:100 dilution.![Western Blot: MSH6 Antibody [NBP1-33151]](https://resources.rndsystems.com/images/products/MSH6-Antibody-Western-Blot-NBP1-33151-img0006.jpg "Western Blot: MSH6 Antibody [NBP1-33151]")
Western Blot: MSH6 Antibody [NBP1-33151]
Western Blot: MSH6 Antibody [NBP1-33151] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract 5% SDS-PAGE gel, antibody dilution 1:1000.![Immunoprecipitation: MSH6 Antibody [NBP1-33151]](https://resources.rndsystems.com/images/products/MSH6-Antibody-Immunoprecipitation-NBP1-33151-img0010.jpg "Immunoprecipitation: MSH6 Antibody [NBP1-33151]")
Immunoprecipitation: MSH6 Antibody [NBP1-33151]
Immunoprecipitation: MSH6 Antibody [NBP1-33151] - MSH6 antibody immunoprecipitates MSH6 protein in IP experiments. IP samples: 293T whole cell extract A. 40 ug 293T whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MSH6 protein by 4 ug MSH6 antibody. 5 % SDS-PAGE. The immunoprecipitated MSH6 protein was detected by MSH6 antibody diluted at 1:1000.Applications for MSH6 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Application Notes
EMSA Assay dependent.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MSH6
Alternate Names
DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein
Gene Symbol
MSH6
Additional MSH6 Products
Product Documents for MSH6 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MSH6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for MSH6 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for MSH6 Antibody - BSA Free
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Q: I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.
A: Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.
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