mu Opioid R/OPRM1 Overexpression Lysate

Novus Biologicals | Catalog # NBP2-04182

Novus Biologicals
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Key Product Details

Species

Human

Applications

Western Blot

Product Summary for mu Opioid R/OPRM1 Overexpression Lysate

mu Opioid R/OPRM1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210383

Accession#: NM_000914

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

44.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for mu Opioid R/OPRM1 Overexpression Lysate

Western Blot: mu Opioid R/OPRM1 Overexpression Lysate [NBP2-04182]

Western Blot: mu Opioid R/OPRM1 Overexpression Lysate [NBP2-04182]

Western Blot: Mu Opioid Receptor Overexpression Lysate (Adult Normal) [NBP2-04182] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mu Opioid Receptor.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: mu Opioid R/OPRM1

The powerful effects of opiate are mediated through the opioid receptors located on the plasma membrane of neurons and other peripheral cells. There are at least 3 types of opioid receptors, mu, kappa, and delta, each with a distinct pharmacologic profile. The mu opioid receptor is the primary site of action for the most commonly used opioids, including morphine, heroin, fentanyl, and methadone.

Long Name

mu Opioid Receptor

Alternate Names

LMOR, MOP, mOpioidR, MOR, MOR1, OPRM1

Gene Symbol

OPRM1

Additional mu Opioid R/OPRM1 Products

Product Documents for mu Opioid R/OPRM1 Overexpression Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for mu Opioid R/OPRM1 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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