Cardiac myosin binding protein C3 (MyBPC3) is a 140 kDa thick filament-associated protein located in the crossbridge region of cardiac muscle sarcomeres. It is composed of eight Ig-like domains and three fibronectin 3-like domains and is known to be a physiological substrate of cAMP-dependent protein kinase. MyBPC3 has a role in sarcomeric structure and in the regulation of cardiac muscle contraction. MyBPC3 is released into the coronary effluent during myocardial infarction. Mutations in MyBPC3 are associated with hypertrophic cardiomyopathy.
MyBPC3 Antibody [Allophycocyanin]
Novus Biologicals | Catalog # AF7199A
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse MyBPC3
Arg998-Lys1100
Accession # O70468
Arg998-Lys1100
Accession # O70468
Specificity
Detects human and mouse MyBPC3 in Western blots and in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for MyBPC3 Antibody [Allophycocyanin]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: MyBPC3
Long Name
Myosin Binding Protein C, Cardiac
Alternate Names
C-protein, cardiac muscle isoform, CMH4, FHC, MYBP-C
Gene Symbol
MYBPC3
Additional MyBPC3 Products
Product Documents for MyBPC3 Antibody [Allophycocyanin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MyBPC3 Antibody [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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