Human/Mouse MyBPC3 Antibody Summary
Accession # O70468
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse MyBPC3 by Western Blot. Western blot shows lysates of mouse heart tissue (adult) and mouse embryonic heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MyBPC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7199) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MyBPC3 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
MyBPC3 (Myosin-binding protein C-cardiac type) is a 140-150 kDa member of the MyBP family, Ig superfamily of proteins. It is expressed in cardiac muscle, and contributes both to myosin filament structure by interacting with light meromysin, and the regulation of contraction by binding to myosin subfragment-2, which results in a reduction of actomyosin ATPase activity. Mouse MyBPC3 is 1270 amino acids (aa) in length. It contains five consecutive C2-type Ig-like domains (aa 151-767), three FN type III repeats (aa 768-958), and two additional C-terminal Ig-like domains (aa 967-1270). There are at least three utilized phosphorylation sites and one Pro‑rich region (aa 100-150). In human, multiple mutations generate variable-length premature truncated forms of MyBPC3. There are two potential isoform variants. One basically shows a six aa substitution for aa 339-344, while a second contains the same six aa substitution coupled to an eight aa extension at the N-terminus. Over aa 998‑1100, mouse MyBPC3 shares 89% and 95% aa identity with human and rat MyBPC3, respectively.
Citation for Human/Mouse MyBPC3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Association of cardiac myosin binding protein-C with the ryanodine receptor channel: putative retrograde regulation?
Authors: PJ Stanczyk, M Seidel, J White, C Viero, CH George, S Zissimopou, FA Lai
J. Cell. Sci., 2018;0(0):.
Sample Types: Cell Lysates
Applications: Western Blot
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