Human/Mouse MyBPC3 Antibody

MyBPC3 (Myosin-binding protein C-cardiac type) is a 140-150 kDa member of the MyBP family, Ig superfamily of proteins. It is expressed in cardiac muscle, and contributes both to myosin filament structure by interacting with light meromysin, and the regulation of contraction by binding to myosin subfragment-2, which results in a reduction of actomyosin ATPase activity. Mouse MyBPC3 is 1270 amino acids (aa) in length. It contains five consecutive C2-type Ig-like domains (aa 151-767), three FN type III repeats (aa 768-958), and two additional C-terminal Ig-like domains (aa 967-1270). There are at least three utilized phosphorylation sites and one Pro‑rich region (aa 100-150). In human, multiple mutations generate variable-length premature truncated forms of MyBPC3. There are two potential isoform variants. One basically shows a six aa substitution for aa 339-344, while a second contains the same six aa substitution coupled to an eight aa extension at the N-terminus. Over aa 998‑1100, mouse MyBPC3 shares 89% and 95% aa identity with human and rat MyBPC3, respectively.