Key Product Details
Species Reactivity
Epitope Tag
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc.
Specificity
This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications for Myc Epitope Tag Antibody
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins. This antibody was tested by ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Suggested applications: IF
Suggested applications: IF
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Myc Epitope Tag
Alternate Names
BHLHE39, Class E basic helix-loop-helix protein 39 (bHLHe39), Myc proto-oncogene protein, Proto-oncogene c-Myc, Transcription factor p64
Additional Myc Epitope Tag Products
Product Documents for Myc Epitope Tag Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Myc Epitope Tag Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Conditions
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- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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