MYF6 Antibody

Novus Biologicals | Catalog # NBP1-52030

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MYF6 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SYRPKQENLEGAD corresponding to internal region according to NP_002460.1.

Epitope

C-SYRPKQENLEGAD

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MYF6 Antibody

Western Blot: MYF6 Antibody [NBP1-52030]

Western Blot: MYF6 Antibody [NBP1-52030]

Western Blot: MYF6 Antibody [NBP1-52030] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MYF6 with MYC tag probed with EB09957 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB09957 (0.5mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Applications for MYF6 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human MYF6 (myc and DYKDDDDK tagged), a band of approx. 37 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MYF6

MYF6 (Myogenic factor 6 or herculin) is a 242 amino acid basic helix-loop-helix DNA binding protein that is involved in muscle differentiation. It induces fibroblasts to differentiate into myoblasts. Defects in MYF6 may be a cause of centronuclear myopathy type 3. It may also play a role in muscular dystrophy, rhabdomyosarcoma, myopathy, centronuclear myopathy, alveolar soft part sarcoma and myasthenia gravis.

Alternate Names

bHLHc4BHLHC4, Class C basic helix-loop-helix protein 4, MRF4, MRF4myogenic factor 6, Muscle-specific regulatory factor 4, Myf-6, myogenic factor 6 (herculin)

Entrez Gene IDs

4618 (Human); 17878 (Mouse); 25714 (Rat)

Gene Symbol

MYF6

UniProt

NP_002460.1 (Human)

Additional MYF6 Products

Product Documents for MYF6 Antibody

Certificate of Analysis

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Product Specific Notices for MYF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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