Human Myogenin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-08156

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant

Sensitivity

0.38 ng/mL

Assay Range

0.63 - 40 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Precision

Intra-Assay Precision (Precision within an assay) %CV 5.76

Inter-Assay Precision (Precision between assays) %CV 6.74

Recovery for Human Myogenin ELISA Kit (Colorimetric)

Recovery

90 - 106%

Linearity

Human Myogenin ELISA Kit (Colorimetric)

Scientific Data Images for Human Myogenin ELISA Kit (Colorimetric)

Human Myogenin ELISA Kit (Colorimetric)

ELISA: Human Myogenin ELISA Kit (Colorimetric) [NBP3-08156] -

ELISA: Human Myogenin ELISA Kit (Colorimetric) [NBP3-08156] - Standard Curve Reference

Kit Contents for Human Myogenin ELISA Kit (Colorimetric)

  • Micro ELISA Plate (Dismountable) - 1 EA, Reference Standard - 2 vials, Concentrated Biotinylated Detection Ab (100X) - 120 uL, Concentrated HRP Conjugate (100X) - 120 uL, Reference Standard & Sample Diluent - 20 mL, Biotinylated Detection Ab Diluent - 14

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Myogenin

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of approx. 34 kDa by SDS-PAGE.

Alternate Names

BHLHC3, MYF4, MYOG

Gene Symbol

MYOG

Additional Myogenin Products

Product Documents for Human Myogenin ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human Myogenin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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