Myosin heavy chain 11 Antibody (SPM201) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48446

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat, Porcine, Bovine, Canine, Chicken, Feline, Guinea Pig, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM201

Format

IHC-Prediluted
View all Myosin heavy chain 11 Primary Antibodies »

Product Specifications

Immunogen

Human uterus extract (Uniprot: P35749)

Localization

Cytoplasmic

Marker

Leiomyosarcoma & Myoepithelial Cell Marker

Specificity

Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Myosin heavy chain 11 Antibody (SPM201) - IHC-Prediluted

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (SPM201) - IHC-Prediluted [NBP2-48446]

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (SPM201) - IHC-Prediluted [NBP2-48446]

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (SPM201) - IHC-Prediluted [NBP2-48446] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with SM-MHC Monoclonal Antibody (SPM201).

Applications for Myosin heavy chain 11 Antibody (SPM201) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 16p13.11 Molecular weight of antigen: 205kDa (MHC-1) and 200kDa (MHC-2) Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Myosin heavy chain 11

The protein encoded by the MYH11 gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. MYH11 functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

AAT4, FAA4, MYH11, MYH11 myosin, heavy chain 11, smooth muscle, SMHC, SMMHC, Smooth muscle myosin heavy chain

Gene Symbol

MYH11

Additional Myosin heavy chain 11 Products

Product Documents for Myosin heavy chain 11 Antibody (SPM201) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for Myosin heavy chain 11 Antibody (SPM201) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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