Q: I would like to know if this item, item number NBP1-00190, is very specific to N-linked glycosyl residues only

A: This antibody recognizes the peptide sequence TTHNLEDHTKLP (AA428-439) and is polyclonal, so it can recognize multiple places on this peptide. There is not a glycosylation site within those residues, so no, it is not specific to N-linked glycosyl residues.

N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

Novus Biologicals | Catalog # NBP1-00190

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all N-Acetylgalactosamine-6-Sulfatase/GALNS Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TTHNLEDHTKLP corresponding to internal region according to NP_000503.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-00190]

Western Blot: N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody [NBP1-00190] - (0.01ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

Application

Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.01 - 0.03 ug/ml

Application Notes

WB: Approx. 60 kDa band observed in human bone marrow and placenta lysates (calculated MW of 58.0 kDa band according to NP_000503.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: N-Acetylgalactosamine-6-Sulfatase/GALNS

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq]

Alternate Names

Chondroitinase, Chondroitinsulfatase, GA6S, Galactose-6-Sulfate Sulfatase, GalNAc6S Sulfatase, GALNS, GAS, MPS4A, NAcetylgalactosamine6Sulfatase

Entrez Gene IDs

2588 (Human)

Gene Symbol

GALNS

UniProt

NP_000503.1 (Human)

Additional N-Acetylgalactosamine-6-Sulfatase/GALNS Products

Product Documents for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

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Certificate of Analysis

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Product Specific Notices for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

Lysosomal Enzymes

Sulfation Regulators

Citations for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody

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