N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81621

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all N-Acetylmannosamine Kinase/GNE Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

Western Blot: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Western Blot: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Western Blot: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621] - Analysis in human cell line A-549.
Immunocytochemistry/ Immunofluorescence: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Immunocytochemistry/ Immunofluorescence: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Immunocytochemistry/Immunofluorescence: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621] - Staining of human skin shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP1-81621] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Applications for N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: N-Acetylmannosamine Kinase/GNE

The protein encoded by the GNE gene is a bifunctional enzyme that monitors and begins biosynthesis of N-acetylneuraminic acid (NeuAc). NeuAC is a precursor of sialic acids and functions in early development as sialylation is a component of cell adhesion, signal transduction, and tumorigencity and metastic behavior of malignant cells. The GNE gene has been linked to various diseases such as: myopathy, dementia, glomerulosclerosis, muscular dystrophy, and neuromuscular disease. This protein creates an enzyme that is rate-limiting in the sialic acid biosynthetic pathway as well as plays a role in amino and nucleotide sugar metabolism as well as CMP-N-acetylneuraminate biosynthesis in eukaryotes. The GNE gene is known to interact with genes CRMP1. ZBTB16, PIK3C2A, KIAA1549, and RIF1 to participate in these pathways. GNE exists is five isoforms: isoform 1: 722 amino acids long; 79 kDA; isoform 2: 753 amino acids long, 83 kDA; isoform 3: 681 amino acids long; 74 kDA; isoform 4: 648 amino acids long, 71 kDA; isoform 5: 612 amino acids long, 66 kDA.

Long Name

Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine Kinase

Alternate Names

DMRV, GLCNE, GNE, IBM2, NAcetylmannosamine Kinase, Uae1

Gene Symbol

GNE

Additional N-Acetylmannosamine Kinase/GNE Products

Product Documents for N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

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Protocols

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