N21-MAX Vitamin A Free Media Supplement (50X)

Catalog # Availability Size / Price Qty
AR012
Synaptotagmin Expression in Neurons Cultured in Media Supplemented with N21-MAX Vitamin A Free Media Supplement.
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Product Details
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N21-MAX Vitamin A Free Media Supplement (50X) Summary

Kit Summary

For controlled neural stem and progenitor cell expansion.

Key Benefits

  • Optimized for neural stem cell expansion
  • Limits retinoic acid production to reduce unwanted NSC differentiation
  • Rigorous quality control testing ensures consistency across experiments
  • Eliminates the need for an astrocyte feeder layer
  • Is nutrient-rich, serum-free, and fully defined
 

Why Culture Neurons in Serum-Free N21-MAX Media Supplement?

The N21-MAX Vitamin A Media Supplement is a serum-free and fully-defined media formulation that is optimized for the consistent health and superior growth of neural stem/progenitor cells. The N21-MAX Vitamin A Free Supplement limits NSC differentiation by reducing unwanted retinoic acid production in cell culture medium. It can also be used to mature and maintain healthy primary neurons in culture.

N21-MAX media supplements were designed to eliminate uncontrolled variables, such as those found in both serum-containing media and in some commercial serum-free neuronal supplements. Use of undefined media supplements can confound neuronal growth and maturation. N21-MAX media supplements contain a cocktail of factors optimized for neural stem cell expansion, neuronal survival, and synaptic maturation. Each lot of N21-MAX Media Supplement is tested on primary hippocampal neurons to ensure consistent performance for the user.

The N21-MAX Vitamin A Free Media Supplement:

  • Is fully defined and quality tested to reduce unwanted experimental variation.
  • Contains factors qualified to the expansion of NSCs in vitro.
  • Limits retinoic acid production to reduce unwanted NSC differentiation.
  • Does not require an astrocyte feeder layer.

N21-MAX Vitamin A Free Media Supplement Components

N21-MAX Vitamin A-Free Media Supplement is optimized for the expansion of neural stem cells in culture. The supplement is supplied as a 50X concentrated solution and contains the following 19 components:

  • Albumin (bovine)
  • L-Carnitine
  • Catalase
  • Corticosterone
  • Ethanolamine
  • Glutathione
  • Galactose
  • Holo-Transferrin
  • Insulin
  • Linoleic Acid
  • Linolenic Acid
  • Lipoic Acid
  • Progesterone
  • Putrescine
  • Selenite
  • Superoxide dismutase
  • Triiodo-L-thyronine
  • D,L-alpha-Tocopherol
  • D,L-alpha-Tocopherol acetate

Supplied in a volume sufficient to supplement 500 mL of media at the recommended concentration. N21-MAX Vitamin A Free Media Supplement is tested for use in Neurobasal Media.

 

Precautions

This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious agents, these reagents should be handled as if capable of transmitting infection.

 

Data Examples

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Synaptotagmin Expression in Neurons Cultured in Media Supplemented with N21-MAX Vitamin A Free Media Supplement. E18 rat hippocampal neurons grown for 21 days in vitro in media supplemented with N21-MAX Vitamin A Free Media Supplement. The cells were stained with Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB4364) followed by the NorthernLights™ (NL)557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue).

Neural stem cells provide an excellent model for research focused on neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. In addition, primary mouse cortical stem cells isolated from E14.5 CD-1 mice are available. Every lot of R&D Systems Cortical Stem Cells is validated for a high level of Nestin expression and the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. Our cortical stem cells are tested to ensure highest quality and lot to lot consistency. Both rat and mouse Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.

To complement the use of primary neural stem cells, we offer a range of supportive products, including culture media which is specifically optimized for use with neural stem cells. We offer kits to promote the in vitro proliferation of neural precursors, and kits to differentiate neural stem cells into dopaminergic neurons or oligodendrocytes. In addition, kits are available which contain panels of antibodies designed to monitor the differentiation and identification of neural precursors, astrocytes, neurons, and oligodendrocytes.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Datasheets

Data Example

Synaptotagmin Expression in Neurons Cultured in Media Supplemented with N21-MAX Vitamin A Free Media Supplement. Synaptotagmin Expression in Neurons Cultured in Media Supplemented with N21-MAX Vitamin A Free Media Supplement.E18 rat hippocampal neurons grown for 21 daysin vitroin media supplemented with N21-MAX Vitamin A Free Media Supplement. The cells were stained with Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB4364) followed by the NorthernLights™ (NL)557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue).

Synaptotagmin Expression in Neurons Cultured in Media Supplemented with N21-MAX Vitamin A Free Media Supplement. E18 rat hippocampal neurons grown for 21 days in vitro in media supplemented with N21-MAX Vitamin A Free Media Supplement. The cells were stained with Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB4364) followed by the NorthernLights™ (NL)557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue).

Assay Procedure

Refer to the product datasheet for complete product details.

 

Briefly, completed N21-MAX Vitamin A Free-supplemented medium is prepared using the following procedure:

  • Dilute the media supplement in basal media
  • Store completed media
  • Use within 2 weeks in the dark
 

 

Reagents Provided

Reagents provided in the N21-MAX Vitamin A Free Media Supplement (Catalog # AR012):

  • Albumin (bovine)
  • L-Carnitine
  • Catalase
  • Corticosterone
  • Ethanolamine
  • Glutathione
  • Galactose
  • Holo-Transferrin
  • Insulin
  • Linoleic Acid
  • Lipoic Acid
  • Progesterone
  • Putrescine
  • Selenite
  • Superoxide dismutase
  • Triiodo-L-thyronine
  • D,L-alpha-Tocopherol
  • D,L-alpha-Tocopherol acetate
 

 

Other Supplies Required

Reagents

  • Basal media (e.g., Neurobasal Media from Invitrogen®, Catalog # 21103-029 or equivalent)
  • L-Glutamine

Materials

  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 2 °C to 8 °C refrigerator
 

 

Protocol Overview

Dilute 50-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) and supplement with 0.5 mM L-glutamine before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

Invitrogen is a registered trademark of Invitrogen Corp.

Reagents Provided

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for N21-MAX Vitamin A Free Media Supplement (50X)

Average Rating: 3.5 (Based on 2 Reviews)

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N21-MAX Vitamin A Free Media Supplement (50X)
By Anonymous on 12/21/2016
Application:

This worked well for neural stem cell expansion.


N21-MAX Vitamin A Free Media Supplement (50X)
By Anonymous on 12/06/2016
Application:

Used for human iPSC differentiation.