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NanoLuc® (Nluc) Luciferase Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB100261U Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB100261U-100UG

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NanoLuc® (Nluc) Luciferase Alexa Fluor® 350-conjugated Antibody Summary

Specificity
Detects NanoLuc® (Nluc) Luciferase in direct ELISAs. In Western blots, no cross-reactivity with Large BiT is observed.
Source
Monoclonal Mouse IgG2a Clone # 965853
Purification
Protein A or G purified
Immunogen
Synthetic peptide for NanoLuc® (Nluc) Luciferase
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Luciferase

NanoLuc® (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. NanoLuc® Binary Technology (NanoBiT™) is a two subunit system based on NanoLuc® luciferase. Large BiT (LgBiT; 17.6kDa) is the large subunit of the NanoBiT® system.

Alternate Names
ec 1.13.12.7; firefly luciferase; Fluc; LuC; Luciferase; luciferin 4 monooxygenase; Luciferin 4-monooxygenase

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