NanoLuc® (Nluc) Luciferase Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of NanoLuc® Luciferase by Western Blot. Western blot shows Recombinant NanoLuc®. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NanoLuc® (Nluc) Luciferase Monoclonal Antibody (Catalog # MAB100261) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NanoLuc® at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
NanoLuc® (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. NanoLuc® Binary Technology (NanoBiT™) is a two subunit system based on NanoLuc® luciferase. Large BiT (LgBiT; 17.6kDa) is the large subunit of the NanoBiT® system.
Product Specific NoticesNanoLuc and NanoBiT are registered trademarks of Promega Corporation.
Citation for NanoLuc® (Nluc) Luciferase Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain
Authors: M Kowalski-J, H Schihada, A Turku, T Huber, TP Sakmar, G Schulte
Science Advances, 2021;7(46):eabj7917.
Sample Types: Cell Lysates
Applications: Western Blot
What is the immunogen for the NanoLuc® (Nluc) Luciferase Antibody, Catalog # MAB100261? Does this antibody recognize LgBit or SmBit?
The immunoogen for MAB100261 is a Synthetic peptide of NanoLuc® (Nluc) Luciferase. For more details on the compound, Promega Corporation should be contacted. NanoBit (LgBit+SmBit) is a derivative of the original product NanoLuc, where portion of the protein is split to get two complementary peptides. MAB100261 detects an epitope on NanoLuc that is not part of LgBit or SmBit. SmBit is only 11 amino acids in length.
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