NanoLuc® (Nluc) Luciferase Antibody

Catalog # Availability Size / Price Qty
Detection of NanoLuc® Luciferase by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products
Reviews (1)

NanoLuc® (Nluc) Luciferase Antibody Summary

Detects NanoLuc® (Nluc) Luciferase in direct ELISAs. In Western blots, no cross-reactivity with Large BiT is observed.
Monoclonal Mouse IgG2A Clone # 965853
Protein A or G purified from hybridoma culture supernatant
Synthetic peptide for NanoLuc® (Nluc) Luciferase
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of NanoLuc® Luciferase antibody by Western Blot. View Larger

Detection of NanoLuc® Luciferase by Western Blot. Western blot shows Recombinant NanoLuc®. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NanoLuc® (Nluc) Luciferase Monoclonal Antibody (Catalog # MAB100261) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NanoLuc® at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Luciferase

NanoLuc® (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. NanoLuc® Binary Technology (NanoBiT™) is a two subunit system based on NanoLuc® luciferase. Large BiT (LgBiT; 17.6kDa) is the large subunit of the NanoBiT® system.

Alternate Names
ec; firefly luciferase; Fluc; LuC; Luciferase; luciferin 4 monooxygenase; Luciferin 4-monooxygenase

Product Datasheets

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Product Specific Notices

NanoLuc and NanoBiT are registered trademarks of Promega Corporation.

Citation for NanoLuc® (Nluc) Luciferase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain
    Authors: M Kowalski-J, H Schihada, A Turku, T Huber, TP Sakmar, G Schulte
    Science Advances, 2021;7(46):eabj7917.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


  1. What is the immunogen for the NanoLuc® (Nluc) Luciferase Antibody, Catalog # MAB100261? Does this antibody recognize LgBit or SmBit?

    • The immunoogen for MAB100261 is a Synthetic peptide of NanoLuc® (Nluc) Luciferase. For more details on the compound, Promega Corporation should be contacted. NanoBit (LgBit+SmBit) is a derivative of the original product NanoLuc, where portion of the protein is split to get two complementary peptides. MAB100261 detects an epitope on NanoLuc that is not part of LgBit or SmBit. SmBit is only 11 amino acids in length.

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for NanoLuc® (Nluc) Luciferase Antibody

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NanoLuc® (Nluc) Luciferase Antibody
By Juan Guzman on 06/08/2020
Application: ICC/IF Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Really nice antibody to work with. It gives you really bright pictures.