Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NAP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 600 and 650 of human TAK1 binding protein 3 (mitogen-activated protein kinase kinase kinase 7 interacting protein 3) using the numbering given in entry NP_690000.2 (GeneID 257397)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NAP1 Antibody - BSA Free

Western Blot: NAP1 Antibody [NBP1-26592]

Western Blot: NAP1 Antibody [NBP1-26592]

Western Blot: NAP1 Antibody [NBP1-26592] - Detection of Human and Mouse TAB3 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from 293T and (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: NAP1 Antibody [NBP1-26592]

Western Blot: NAP1 Antibody [NBP1-26592]

Western Blot: NAP1 Antibody [NBP1-26592] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. TAB3 was also immunoprecipitated by rabbit anti-TAB3 antibody NBP1-26593.
Immunoprecipitation: NAP1 Antibody [NBP1-26592]

Immunoprecipitation: NAP1 Antibody [NBP1-26592]

Immunoprecipitation: NAP1 Antibody [NBP1-26592] - Detection of human TAB3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 (lot NBP1-26592-2) used for IP at 6 ug per reaction. TAB3 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-26592-1). For blotting immunoprecipitated TAB3, NBP1-26592 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 60 seconds.

Applications for NAP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NAP1

TAK1-binding protein 3 (TAB3) is a binding and regulatory subunit of the transforming growth factor-beta-activated kinase 1 (TAK1). TAB3 functions as an adaptor that mediates IL1 and TNF signaling pathways. Additionally it plays a role in the activation of the NF-kappaB pathway by functioning as receptors that bind polyubiquitin chains for the regulation of kinase activity.

Alternate Names

CG5330, Dmel\CG5330, dNap, dNAP1, dNAP-1, MAP3K7IP3, MGC45404, mitogen-activated protein kinase kinase kinase 7 interacting protein 3, Mitogen-activated protein kinase kinase kinase 7-interacting protein 3, NAP1, Nap-1, NF-kappa-B-activating protein 1, NFkB activating protein 1, p56/dCAF-4, TAB-3, TAK1-binding protein 3, TGF-beta activated kinase 1/MAP3K7 binding protein 3, TGF-beta-activated kinase 1 and MAP3K7-binding protein 3, TGF-beta-activated kinase 1-binding protein 3

Entrez Gene IDs

257397 (Human)

Gene Symbol

TAB3

UniProt

NP_690000.2 (Human)

Additional NAP1 Products

Product Documents for NAP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NAP1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review NAP1 Antibody - BSA Free and earn rewards!

Have you used NAP1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies