NCK1 Antibody (1A1) - Azide and BSA Free

Novus Biologicals | Catalog # H00004690-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A1

Format

Azide and BSA Free
View all NCK1 Primary Antibodies »

Product Specifications

Immunogen

NCK1 (AAH06403, 185 a.a. ~ 294 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEM

Localization

Cytoplasmic

Specificity

NCK1 - NCK adaptor protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NCK1 Antibody (1A1) - Azide and BSA Free

Western Blot: NCK1 Antibody (1A1) [H00004690-M01]

Western Blot: NCK1 Antibody (1A1) [H00004690-M01]

Western Blot: NCK1 Antibody (1A1) [H00004690-M01] - Analysis of NCK1 expression in transfected 293T cell line by NCK1 monoclonal antibody (M01), clone 1A1.Lane 1: NCK1 transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.
Western Blot: NCK1 Antibody (1A1) [H00004690-M01]

Western Blot: NCK1 Antibody (1A1) [H00004690-M01]

Western Blot: NCK1 Antibody (1A1) [H00004690-M01] - NCK1 monoclonal antibody (M01), clone 1A1 Analysis of NCK1 expression in Hela S3 NE.
ELISA: NCK1 Antibody (1A1) [H00004690-M01]

ELISA: NCK1 Antibody (1A1) [H00004690-M01]

ELISA: NCK1 Antibody (1A1) [H00004690-M01] - Detection limit for recombinant GST tagged NCK1 is approximately 0.1ng/ml as a capture antibody.

Applications for NCK1 Antibody (1A1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NCK1

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS.

Long Name

Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1

Alternate Names

cytoplasmic protein NCK1, MGC12668, NCK adaptor protein 1melanoma NCK protein, Nck-1, NCKalpha, NCKNCK tyrosine kinase, non-catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha

Entrez Gene IDs

4690 (Human)

Gene Symbol

NCK1

OMIM

600508 (Human)

UniProt

AAH06403 (Human)

Additional NCK1 Products

Product Documents for NCK1 Antibody (1A1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NCK1 Antibody (1A1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases
PDGF Family

Citations for NCK1 Antibody (1A1) - Azide and BSA Free

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail