NCOA4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-18136
![Western Blot: NCOA4 AntibodyBSA Free [NBP3-18136]](https://resources.rndsystems.com/images/products/NCOA4-Antibody---BSA-Free-Western-Blot-NBP3-18136-img0003.jpg "Western Blot: NCOA4 AntibodyBSA Free [NBP3-18136]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Canine (100%), Equine (100%), Monkey (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NCOA4 Antibody - BSA Free
Western Blot: NCOA4 AntibodyBSA Free [NBP3-18136]
Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Rat Tissues. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.![Immunocytochemistry/ Immunofluorescence: NCOA4 Antibody - BSA Free [NBP3-18136]](https://resources.rndsystems.com/images/products/NCOA4-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-18136-img0004.jpg "Immunocytochemistry/ Immunofluorescence: NCOA4 Antibody - BSA Free [NBP3-18136]")
Immunocytochemistry/ Immunofluorescence: NCOA4 Antibody - BSA Free [NBP3-18136]
Immunocytochemistry/Immunofluorescence: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with NBP3-18136 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).![Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136]](https://resources.rndsystems.com/images/products/NCOA4-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP3-18136-img0006.jpg "Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136]")
Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136]
Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.![Western Blot: NCOA4 AntibodyBSA Free [NBP3-18136]](https://resources.rndsystems.com/images/products/NCOA4-Antibody---BSA-Free-Western-Blot-NBP3-18136-img0001.jpg "Western Blot: NCOA4 AntibodyBSA Free [NBP3-18136]")
Western Blot: NCOA4 AntibodyBSA Free [NBP3-18136]
Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 0.5 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.![Western Blot: NCOA4 AntibodyBSA Free [NBP3-18136]](https://resources.rndsystems.com/images/products/NCOA4-Antibody---BSA-Free-Western-Blot-NBP3-18136-img0002.jpg "Western Blot: NCOA4 AntibodyBSA Free [NBP3-18136]")
Western Blot: NCOA4 AntibodyBSA Free [NBP3-18136]
Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Mouse Tissues. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.![Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136]](https://resources.rndsystems.com/images/products/NCOA4-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP3-18136-img0005.jpg "Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136]")
Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136]
Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.![Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136]](https://resources.rndsystems.com/images/products/NCOA4 Antibody - BSA Free-Immunohistochemistry-Paraffin-NBP3-18136-img0007.jpg "Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136]")
Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136]
Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.Applications for NCOA4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
1 ug/ml
Western Blot
0.5-1 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NCOA4
Alternate Names
Androgen receptor coactivator 70 kDa protein, Androgen receptor-associated protein of 70 kDa, ARA70RFGret fused, DKFZp762E1112, ELE1NCoA-4, nuclear receptor coactivator 4,70 kDa AR-activator, PTC3RET-activating gene ELE1, Ret-activating protein ELE1,70 kDa androgen receptor coactivator
Gene Symbol
NCOA4
Additional NCOA4 Products
Product Documents for NCOA4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NCOA4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for NCOA4 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review NCOA4 Antibody - BSA Free and earn rewards!
Have you used NCOA4 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...