NCOR1 Antibody

Novus Biologicals | Catalog # NB100-58824

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NCOR1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1525 and 1575 of human nuclear receptor co-repressor 1 using the numbering given in entry NP_006302.2 (GeneID 9611).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB200-347

Scientific Data Images for NCOR1 Antibody

Western Blot: NCOR1 Antibody [NB100-58824]

Western Blot: NCOR1 Antibody [NB100-58824]

Western Blot: NCOR1 Antibody [NB100-58824] - Whole cell lysate (50 ug) from HEK293T, K-562, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.
Chromatin Immunoprecipitation Sequencing: NCOR1 Antibody [NB100-58824] - Localization of NCoR Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-NCoR antibody NB100-58824 and analyzed by DNA sequencing. The figure illustrates the peak distribution of NCoR binding within a 650 Kb region of the human X chromosome as detected using anti-NCoR antibody NB100-58824. ChIP-seq validation performed by Diogenode, Denville, NJ.
Immunoprecipitation: NCOR1 Antibody [NB100-58824]

Immunoprecipitation: NCOR1 Antibody [NB100-58824]

Immunoprecipitation: NCOR1 Antibody [NB100-58824] - Detection of Human NCOR on HeLa whole cell lysate using NB100-5882. NCOR was also immunoprecipitated by rabbit anti-NCOR antibody NB100-58825.

Applications for NCOR1 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

10 ug

Chromatin Immunoprecipitation Sequencing

2 ug

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
ChIP-Seq 10 ug as per Ram O et al., Cell. 2011 Dec 23;147(7):1628-39.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCOR1

This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined.

Long Name

Nuclear Receptor Co-repressor 1

Alternate Names

KIAA1047, N-CoR, TRAC1

Entrez Gene IDs

9611 (Human)

Gene Symbol

NCOR1

UniProt

O75376 (Human)

Additional NCOR1 Products

Product Documents for NCOR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NCOR1 Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB200-347

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail