NDUFA8 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-93802

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all NDUFA8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 33-172 of human NDUFA8 (NP_055037.1). GAQCDKPNKEFMLCRWEEKDPRRCLEEGKLVNKCALDFFRQIKRHCAEPFTEYWTCIDYTGQQLFRHCRKQQAKFDECVLDKLGWVRPDLGELSKVTKVKTDRPLPENPYHSRPRPDPSPEIEGDLQPATHGSRFYFWTK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDUFA8 Antibody - Azide and BSA Free

Western Blot: NDUFA8 AntibodyAzide and BSA Free [NBP2-93802]

Western Blot: NDUFA8 AntibodyAzide and BSA Free [NBP2-93802]

Western Blot: NDUFA8 Antibody [NBP2-93802] - Analysis of 200ug extracts of 293T cells, using NDUFA8 at a dilition of 1:1000.
Immunocytochemistry/ Immunofluorescence: NDUFA8 Antibody - Azide and BSA Free [NBP2-93802]

Immunocytochemistry/ Immunofluorescence: NDUFA8 Antibody - Azide and BSA Free [NBP2-93802]

Immunocytochemistry/Immunofluorescence: NDUFA8 Antibody [NBP2-93802] - Immunofluorescence analysis of L929 cells using NDUFA8 Rabbit pAb (NBP2-93802) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: NDUFA8 AntibodyAzide and BSA Free [NBP2-93802]

Western Blot: NDUFA8 AntibodyAzide and BSA Free [NBP2-93802]

Western Blot: NDUFA8 Antibody [NBP2-93802] - Analysis of extracts of various cell lines, using NDUFA8 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: NDUFA8 Antibody - Azide and BSA Free [NBP2-93802]

Immunocytochemistry/ Immunofluorescence: NDUFA8 Antibody - Azide and BSA Free [NBP2-93802]

Immunocytochemistry/Immunofluorescence: NDUFA8 Antibody [NBP2-93802] - Immunofluorescence analysis of H9C2 cells using NDUFA8 Rabbit pAb (NBP2-93802) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for NDUFA8 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDUFA8

NDUFA8 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternate Names

CI-19kD, CI-PGIV, complex I PGIV subunit, Complex I-19kD, Complex I-PGIV, MGC793, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8 (19kD, PGIV), NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, NADH:ubiquinone oxidoreductase PGIV subunit, NADH-ubiquinone oxidoreductase 19 kDa subunit, PGIV

Gene Symbol

NDUFA8

Additional NDUFA8 Products

Product Documents for NDUFA8 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NDUFA8 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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