NDUFB5 Overexpression Lysate
Novus Biologicals | Catalog # NBL1-13553
![Western Blot: NDUFB5 Overexpression Lysate [NBL1-13553]](https://resources.rndsystems.com/images/products/NDUFB5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13553-img0002.jpg "Western Blot: NDUFB5 Overexpression Lysate [NBL1-13553]")
Key Product Details
Species
Applications
Product Summary for NDUFB5 Overexpression Lysate
NDUFB5 Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC202714
Accession#: NM_002492
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).
Product Specifications
Application Notes
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Scientific Data Images for NDUFB5 Overexpression Lysate
Western Blot: NDUFB5 Overexpression Lysate [NBL1-13553]
Western Blot: NDUFB5 Overexpression Lysate (Adult Normal) [NBL1-13553] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDUFB5.Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: NDUFB5
Alternate Names
Gene Symbol
Additional NDUFB5 Products
Product Documents for NDUFB5 Overexpression Lysate
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NDUFB5 Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for NDUFB5 Overexpression Lysate
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Q: How come the vector transfected control has no band? There should be endogenous level of NDUFB5 in Hek293 cells.
A: We test most of our over-expression lysates with anti-DDK to ensure the protein is at the correct size. NDUFB5 will have endogenous levels in the HEK293 lysate, and you may see a band in the empty vector lane, but you should see an increase in intensity in the over-expression lysate lane.