NDUFB9 Antibody (OTI13H11)

Novus Biologicals | Catalog # NBP2-46126

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI13H11
View all NDUFB9 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 3-179 of human NDUFB9(NP_0049906) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDUFB9 Antibody (OTI13H11)

Western Blot: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Western Blot: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Western Blot: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB9.
Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Flow Cytometry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Flow Cytometry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Jurkat cells, using NDUFB9 antibody,(Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Western Blot: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Western Blot: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of extracts (10ug) from 6 different cell lines by using NDUFB9 monoclonal antibody.
Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Immunohistochemistry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Flow Cytometry: NDUFB9 Antibody (OTI13H11) [NBP2-46126]

Flow Cytometry: NDUFB9 Antibody (OTI13H11) [NBP2-46126] - Analysis of Hela cells, using NDUFB9 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Applications for NDUFB9 Antibody (OTI13H11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:200 - 1:1000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDUFB9

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Alternate Names

B22, CI-B22, complex I B22 subunit, Complex I-B22, FLJ22885, LYR motif-containing protein 3, LYRM3DKFZp566O173, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9, NADH-ubiquinone oxidoreductase B22 subunit, UQOR22NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (22kD, B22)

Gene Symbol

NDUFB9

Additional NDUFB9 Products

Product Documents for NDUFB9 Antibody (OTI13H11)

Certificate of Analysis

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Product Specific Notices for NDUFB9 Antibody (OTI13H11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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