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NECA | CAS No. 35920-39-9 | Non-selective Adenosine Receptor Agonists
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Description: High affinity adenosine agonist
Alternative Names: 5'-N-Ethylcarboxamidoadenosine

Chemical Name: 1-(6-Amino-9H-purin-9-yl)-1-deoxy-N-ethyl-β-D-ribofuranuronamide

Purity: ≥98%

Product Details
Citations (25)
Supplemental Products

Biological Activity

NECA is a high affinity adenosine receptor agonist (Ki values are 6.2, 14, and 20 nM for human A3, A1 and A2A receptors respectively; EC50 = 2.4 μM for human A2B). Inhibits platelet aggregation and is centrally active in vivo.

Technical Data

Soluble to 40 mM in DMSO
Store at +4°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Hypoxia inducible factor HIF-1 promotes myeloid-derived suppressor cells accumulation through ENTPD2/CD39L1 in hepatocellular carcinoma
    DK Chiu, AP Tse, IM Xu, J Di Cui, RK Lai, LL Li, HY Koh, FH Tsang, LL Wei, CM Wong, IO Ng, CC Wong
    Nat Commun, 2017;8(1):517.
  2. Adenosine augments IL-10-induced STAT3 signaling in M2c macrophages.
    Koscso B, Csoka B, Kokai E, Nemeth Z, Pacher P, Virag L, Leibovich S, Hasko G
    J Leukoc Biol, 2013;94(6):1309-15.
  3. IFN-gamma Prevents Adenosine Receptor (A2bR) Upregulation To Sustain the Macrophage Activation Response.
    Cohen H, Ward A, Hamidzadeh K, Ravid K, Mosser D
    J Immunol, 2015;195(8):3828-37.
  4. 5'-N-ethylcarboxamidoadenosine: a potent inhibitor of human platelet aggregation.
    Cusack and Hourani
    Br.J.Pharmacol., 1981;72:443
  5. Adenosine receptors and their ligands.
    Naunyn Schmiedebergs Arch.Pharmacol., 2000;362:382
  6. Adenosine agonists CGS21680 and NECA inhibit the initiation of cocaine self-administration.
    Knapp et al.
    Pharmacol.Biochem.Behav., 2001;68:797

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Citations for NECA

The citations listed below are publications that use Tocris products. Selected citations for NECA include:

25 Citations: Showing 1 - 10

  1. Inosine induces stemness features in CAR-T cells and enhances potency.
    Authors: Klysz Et al.
    Cancer Cell  2024;42:266
  2. A Differential Hypofunctionality of Gαi Proteins Occurs in Adolescent Idiopathic Scoliosis and Correlates with the Risk of Disease Progression.
    Authors: Akoume Et al.
    Sci Rep  2019;9:10074
  3. Adenosine binds predominantly to adenosine receptor A1 subtype in astrocytes and mediates an immunosuppressive effect.
    Authors: Liu Et al.
    Brain Res  2018;1700:47
  4. Erythrocytes retain hypoxic adenosine response for faster acclimatization upon re-ascent.
    Authors: Song
    Nat Commun  2017;8:14108
  5. Prostaglandin E2-mediated adenosinergic effects on CD14+ cells: Self-amplifying immunosuppression in cancer.
    Authors: Al-Taei Et al.
    Oncoimmunology  2017;6:e1268308
  6. Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes.
    Authors: Salimu Et al.
    J Extracell Vesicles  2017;6:1368823
  7. Critical role for adenosine receptor A2a in β-cell proliferation.
    Authors: Schulz Et al.
    Mol Metab  2016;5:1138
  8. A2A adenosine receptor modulates drug efflux transporter P-glycoprotein at the blood-brain barrier.
    Authors: Kim and Bynoe
    J Clin Invest  2016;126:1717
  9. CD73-generated adenosine is critical for immune regulation during Toxoplasma gondii infection.
    Authors: Mahamed Et al.
    Infect Immun  2015;83:721
  10. A2A Adenosine Receptor Regulates the Human Blood-Brain Barrier Permeability.
    Authors: Kim and Bynoe
    Am J Respir Cell Mol Biol  2015;52:664
  11. Arginine 199 and leucine 208 have key roles in the control of adenosine A2A receptor signalling function.
    Authors: Bertheleme Et al.
    PLoS One  2014;9:e89613
  12. Positive allosteric modulation of the adenosine A2a receptor attenuates inflammation.
    Authors: Welihinda and Amento
    J Inflamm (Lond)  2014;11:37
  13. Enhanced brain distribution of modified aspartoacylase.
    Authors: Poddar Et al.
    Mol Genet Metab  2014;113:219
  14. NTPDase1 activity attenuates microglial phagocytosis.
    Authors: Bulavina Et al.
    Purinergic Signal  2013;9:199
  15. Macrophage A2A adenosinergic receptor modulates oxygen-induced augmentation of murine lung injury.
    Authors: Aggarwal Et al.
    PLoS One  2013;48:635
  16. Autocrine adenosine signaling promotes regulatory T cell-mediated renal protection.
    Authors: Kinsey Et al.
    J Am Soc Nephrol  2012;23:1528
  17. Interleukin-6 contributes to inflammation and remodeling in a model of adenosine mediated lung injury.
    Authors: Pedroza Et al.
    J Clin Immunol  2011;6:e22667
  18. Disparity in FcηRI-induced degranulation of primary human lung and skin mast cells exposed to adenosine.
    Authors: Gomez Et al.
    Nature  2011;31:479
  19. Adenosine receptor signaling modulates permeability of the blood-brain barrier.
    Authors: Carman Et al.
    J Neurosci  2011;31:13272
  20. CD73 on tumor cells impairs antitumor T-cell responses: a novel mechanism of tumor-induced immune suppression.
    Authors: Jin Et al.
    Cancer Res  2010;70:2245
  21. Adaptation of NS cells growth and differentiation to high-throughput screening-compatible plates.
    Authors: Garavaglia Et al.
    BMC Neurosci  2010;11:7
  22. Adenosine A2A and A2B receptors work in concert to induce a strong protection against reperfusion injury in rat hearts.
    Authors: Xi Et al.
    J Mol Cell Cardiol  2009;47:684
  23. Adenosine-evoked hyperpolarization of retinal ganglion cells is mediated by G-protein-coupled inwardly rectifying K+ and small conductance Ca2+-activated K+ channel activation.
    Authors: Clark Et al.
    J Neurosci  2009;29:11237
  24. Calcium increases in retinal glial cells evoked by light-induced neuronal activity.
    Authors: Newman
    Mol Neurobiol  2005;25:5502


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