NECAB1 Antibody (OTI4G8)

Novus Biologicals | Catalog # NBP2-00480

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4G8
View all NECAB1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NECAB1 (NP_071746) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NECAB1 Antibody (OTI4G8)

Western Blot: NECAB1 Antibody (4G8) [NBP2-00480] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.
Flow Cytometry: NECAB1 Antibody (OTI4G8) [NBP2-00480]

Flow Cytometry: NECAB1 Antibody (OTI4G8) [NBP2-00480]

Flow Cytometry: NECAB1 Antibody (4G8) [NBP2-00480] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NECAB1 antibody, and then analyzed by flow cytometry.
Flow Cytometry: NECAB1 Antibody (OTI4G8) [NBP2-00480]

Flow Cytometry: NECAB1 Antibody (OTI4G8) [NBP2-00480]

Flow Cytometry: NECAB1 Antibody (4G8) [NBP2-00480] - Analysis of Hela cells, using anti-NECAB1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: NECAB1 Antibody (OTI4G8) [NBP2-00480]

Flow Cytometry: NECAB1 Antibody (OTI4G8) [NBP2-00480]

Flow Cytometry: NECAB1 Antibody (4G8) [NBP2-00480] - Analysis of Jurkat cells, using anti-NECAB1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for NECAB1 Antibody (OTI4G8)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.59 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NECAB1

The NECAB1 gene codes for a N-terminal EF-hand calcium-binding protein 1 that in isoform 1 is 351 amino acids long and 40 kDA and in isoform 2 is 100 amino acids long at 11 kDA. NECAB1 has been linked to diseases such as seizures, schizophrenia, alzheimer's disease, motor neuron disease, neuronitis, and neurodegenerative disease.

Alternate Names

EF hand calcium binding protein 1, EFCBP1, EF-hand calcium binding protein 1, EF-hand calcium-binding protein 1, neuronal calcium binding protein, Neuronal calcium-binding protein 1, N-terminal EF-hand calcium binding protein 1, N-terminal EF-hand calcium-binding protein 1, STIP-1, synaptotagmin interacting protein 1

Entrez Gene IDs

64168 (Human)

Gene Symbol

NECAB1

Additional NECAB1 Products

Product Documents for NECAB1 Antibody (OTI4G8)

Certificate of Analysis

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Product Specific Notices for NECAB1 Antibody (OTI4G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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