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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
between 425 and 475
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NEDD4L Antibody
Western Blot:[NBP3-29856]-
Western Blot:[NBP3-29856]-Detection of human and mouse NEDD4L by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa, HEK293T, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NEDD4L antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 3 µg/mg lysate. NEDD4L was also immunoprecipitated by rabbit anti-NEDD4L antibodies and, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 second and 10 seconds.Applications for NEDD4L Antibody
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-buffered Saline 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NEDD4L
Alternate Names
E3 ubiquitin-protein ligase NEDD4-like, EC 6.3.2, EC 6.3.2.-, FLJ33870, hNedd4-2, KIAA0439ubiquitin-protein ligase NEDD4-like, NEDD4.2, Nedd4-2, NEDL3, neural precursor cell expressed, developmentally down-regulated 4-like, neural precursor cell expressed, developmentally down-regulated gene 4-like, RSP5ubiquitin-protein ligase Rsp5
Gene Symbol
NEDD4L
Additional NEDD4L Products
Product Documents for NEDD4L Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NEDD4L Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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