NEK7 Antibody (HL1348) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25575

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (93%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1348 expressed in HEK293

Format

Azide and BSA Free
View all NEK7 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NEK7. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (86%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEK7 Antibody (HL1348) - Azide and BSA Free

NEK7 Antibody (HL1348) - Azide and BSA Free

Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] -

Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - Wild-type (WT) and NEK7 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NEK7 Antibody (HL1348) - Azide and BSA Free

Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] -

Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NEK7 Antibody (HL1348) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] -

Immunocytochemistry/Immunofluorescence: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI.
NEK7 Antibody (HL1348) - Azide and BSA Free

Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] -

Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NEK7 Antibody (HL1348) - Azide and BSA Free

Western Blot: NEK7 Antibody (HL1348) [NBP3-25575] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for NEK7 Antibody (HL1348) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEK7

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM]

Alternate Names

EC 2.7.11.1, Never in mitosis A-related kinase 7, NIMA (never in mitosis gene a)-related kinase 7, NimA-related protein kinase 7, serine/threonine-protein kinase Nek7

Gene Symbol

NEK7

Additional NEK7 Products

Product Documents for NEK7 Antibody (HL1348) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NEK7 Antibody (HL1348) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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