Neomycin phosphotransferase II Antibody (4B4D1)

Novus Biologicals | Catalog # NB110-60487

Novus Biologicals
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Key Product Details

Species Reactivity

Bacteria, Non-species specific

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 4B4D1
View all Neomycin phosphotransferase II Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of Neomycin phosphotransferase II expressed in E. Coli.

Reactivity Notes

Transfected or transformed cells / organisms (plants, animals) carrying NPTII gene encoding neomycin phosphotransferase II enzyme. Escherichia coli (E. coli).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Novus Biologicals Mouse Neomycin phosphotransferase II Antibody (4B4D1) (NB110-60487) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Neomycin phosphotransferase II Antibody (4B4D1)

Western Blot: Neomycin phosphotransferase II Antibody (4B4D1)BSA Free [NB110-60487]

Western Blot: Neomycin phosphotransferase II Antibody (4B4D1)BSA Free [NB110-60487]

Western Blot: Neomycin phosphotransferase II Antibody (4B4D1) [NB110-60487] - Figure 1: Western blot analysis using anti-human NPT monoclonal antibody against recombinant NPT expressed in E. Coli.

Applications for Neomycin phosphotransferase II Antibody (4B4D1)

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neomycin phosphotransferase II

Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.

Alternate Names

NPT2, NPTII

Additional Neomycin phosphotransferase II Products

Product Documents for Neomycin phosphotransferase II Antibody (4B4D1)

Certificate of Analysis

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Product Specific Notices for Neomycin phosphotransferase II Antibody (4B4D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Neomycin phosphotransferase II Antibody (4B4D1)

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