NET1 Antibody (CL3063) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43818
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL3063
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P23975, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for NET1 Antibody (CL3063) - Azide and BSA Free
![NET1 Antibody (CL3063) - Azide and BSA Free Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]](https://resources.rndsystems.com/images/products/nbp3-43818_mouse-net1-mab-cl3063-azide-and-bsa-free-282202512394444.jpg "Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]")
Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]
Staining of human kidney shows no positivity in cells in tubules or glomeruli as expected.![NET1 Antibody (CL3063) - Azide and BSA Free Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]](https://resources.rndsystems.com/images/products/nbp3-43818_mouse-net1-mab-cl3063-azide-and-bsa-free-28220251315459.jpg "Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]")
Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]
Staining of human placenta shows weak membranous positivity in trophoblastic cells.![NET1 Antibody (CL3063) - Azide and BSA Free Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]](https://resources.rndsystems.com/images/products/nbp3-43818_mouse-net1-mab-cl3063-azide-and-bsa-free-28220251375696.jpg "Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]")
Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]
Staining of human prostate shows strong positivity in sympathetic peripheral nerves.![NET1 Antibody (CL3063) - Azide and BSA Free Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]](https://resources.rndsystems.com/images/products/nbp3-43818_mouse-net1-mab-cl3063-azide-and-bsa-free-28220251353273.jpg "Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]")
Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]
Staining of human locus coeruleus shows strong cytoplasmic positivity in noradrenaline neurons.![NET1 Antibody (CL3063) - Azide and BSA Free Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]](https://resources.rndsystems.com/images/products/nbp3-43818_mouse-net1-mab-cl3063-azide-and-bsa-free-282202513314676.jpg "Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]")
Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]
Staining of mouse brain shows strong positivity in noradrenergic fibers in the cerebral cortex.![NET1 Antibody (CL3063) - Azide and BSA Free Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]](https://resources.rndsystems.com/images/products/nbp3-43818_mouse-net1-mab-cl3063-azide-and-bsa-free-28220251335844.jpg "Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]")
Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]
Staining of mouse brain shows strong positivity in noradrenergic fibers in the hippocampus.![NET1 Antibody (CL3063) - Azide and BSA Free Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]](https://resources.rndsystems.com/images/products/nbp3-43818_mouse-net1-mab-cl3063-azide-and-bsa-free-28220251333237.jpg "Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]")
Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]
Staining of rat pons shows strong positivity in noradrenaline neurons in the locus coeruleus.![NET1 Antibody (CL3063) - Azide and BSA Free Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]](https://resources.rndsystems.com/images/products/nbp3-43818_mouse-net1-mab-cl3063-azide-and-bsa-free-282202513332332.jpg "Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]")
Immunohistochemistry-Paraffin: NET1 Antibody [NBP3-43818]
Staining of rat cerebellum shows strong positivity in noradrenergic fibers.Applications for NET1 Antibody (CL3063) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: NET1
Alternate Names
ARHGEF8neuroepithelial cell-transforming gene 1 protein, guanine nucleotide regulatory protein (oncogene), NET1A, neuroepithelial cell transforming 1, neuroepithelial cell transforming gene 1, neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, Proto-oncogene p65 Net1, Rho guanine nucleotide exchange factor (GEF) 8, Rho guanine nucleotide exchange factor 8, small GTP-binding protein regulator
Gene Symbol
NET1
Additional NET1 Products
Product Documents for NET1 Antibody (CL3063) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NET1 Antibody (CL3063) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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