Netrin-1 Antibody (JE38-87)

Western Blot Shows Netrin-1 Specificity Using Knockout Cell Line.

Western blot shows cell culture media from MCF7 parental cell line and Netrin-1 knockout MCF7 cell line (KO). Nitrocellulose membrane was probed with Netrin-1 Antibody (JE38-87) (Catalog # NBP3-32643) followed by HRP-conjugated secondary antibody. A specific band was detected for Netrin-1 at approximately 67.7 kDa (as indicated) in the parental MCF7 cell line, but is not detectable in knockout MCF7 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Detection of Netrin-1 by Immunoprecipitation.

MCF7 cell line cell culture media were prepared and immunoprecipitation was performed using 2.0 μg of Netrin-1 Antibody (JE38-87) (Catalog # NBP3-32643) pre-coupled to Dynabeads Protein A. Immunoprecipitated Netrin-1 was detected in Western Blot with a rabbit Netrin-1 antibody used at 1/2000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot: Netrin-1 Antibody (JE38-87) [NBP3-32643]

Western Blot: Netrin-1 Antibody (JE38-87) [NBP3-32643] - Western blot analysis of Netrin-1 on different lysates with Rabbit anti-Netrin-1 antibody (NBP3-32643) at 1/1,000 dilution.

Lane 1: Human small intestine tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Mouse heart tissue lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32643) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.