Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-14029

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-NEUROG3-1E10

Format

Azide and BSA Free
View all Neurogenin-3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human full-lengthNeurogenin-3protein (Uniprot: Q9Y4Z2)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13987).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free

Protein Array: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free [NBP3-14029]

Protein Array: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free [NBP3-14029]

Protein Array: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free [NBP3-14029] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Neurogenin-3 Antibody (PCRP-NEUROG3-1E10).
Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free [NBP3-14029] -

Immunocytochemistry/ Immunofluorescence: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free [NBP3-14029] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free [NBP3-14029] -

Immunocytochemistry/ Immunofluorescence: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free [NBP3-14029] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free Flow Cytometry: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free [NBP3-14029] -

Flow Cytometry: Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free [NBP3-14029] -

Flow cytometric analysis of PFA-fixed HeLa cells. Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications for Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunoprecipitation

1-2 ug per 100-500 ug protein
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Neurogenin-3

Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm (Gradwohl et al., 2000 [PubMed 10677506]).[supplied by OMIM]

Alternate Names

Atoh5, Math4B, NEUROG3, Neurogenin3, Relax

Gene Symbol

NEUROG3

Additional Neurogenin-3 Products

Product Documents for Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Neurogenin-3 Antibody (PCRP-NEUROG3-1E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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