Neuron-specific beta-III Tubulin APC-conjugated Antibody

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Detection of  beta ‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry.
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Citations (1)
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Neuron-specific beta-III Tubulin APC-conjugated Antibody Summary

Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in flow cytometry.
Monoclonal Mouse IgG2A Clone # TuJ-1
Protein A or G purified from hybridoma culture supernatant
Rat brain-derived microtubules
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of  beta -III Tubulin antibody in Rat Cortical Stem Cells antibody by Flow Cytometry. View Larger

Detection of beta ‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry. Eight-day differentiated Rat cortical stem cells was stained with Mouse Anti-beta -III Tubulin APC-conjugated Monoclonal Antibody (Catalog # IC1195A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: beta-III Tubulin

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium.

Entrez Gene IDs
10381 (Human)
Alternate Names
Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen

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Citation for Neuron-specific beta-III Tubulin APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Characterization of an induced pluripotent stem cell line (IMBPASi001-A) derived from fibroblasts of a patient affected by Wolfram Syndrome
    Authors: DP Grzela, B Marciniak, L Pulaski
    Stem Cell Res, 2020-06-01;46(0):101858.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC


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