Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # TuJ-1
View all beta-III Tubulin Primary Antibodies »

Product Specifications

Immunogen

Rat brain-derived microtubules

Specificity

Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Neuron-specific beta ‑III Tubulin APC‑conjugated Antibody

Detection of beta ‑III Tubulin in A172 cells by Flow Cytometry

A172 cells were stained with Mouse Anti-Neuron-specific beta ‑III Tubulin APC‑conjugated Monoclonal Antibody (Catalog # IC1195A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.
Detection of beta -III Tubulin antibody in Rat Cortical Stem Cells antibody by Flow Cytometry.

Detection of beta ‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry.

Eight-day differentiated Rat cortical stem cells was stained with Mouse Anti-beta -III Tubulin APC-conjugated Monoclonal Antibody (Catalog # IC1195A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Neuron-specific beta ‑III Tubulin APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: A172 human glioblastoma cell line and Eight-day differentiated Rat cortical stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: beta-III Tubulin

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Entrez Gene IDs

10381 (Human)

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for Neuron-specific beta ‑III Tubulin APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Neuron-specific beta ‑III Tubulin APC‑conjugated Antibody

For research use only

Citations for Neuron-specific beta ‑III Tubulin APC‑conjugated Antibody

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