Neuron-specific beta ‑III Tubulin PerCP‑conjugated Antibody

Detection of beta ‑III Tubulin in A172 cells by Flow Cytometry

A172 cells were stained with Mouse Anti-Neuron-specific beta ‑III Tubulin PerCP‑conjugated Monoclonal Antibody (Catalog # IC1195C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.

Detection of beta ‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry.

Eight-day differentiated Rat cortical stem cells were stained with Mouse Anti-Neuron-specific beta -III Tubulin PerCP-conjugated Monoclonal Antibody (IC1195C, filled histogram) or isotype control antibody (IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human beta-III Tubulin by Flow Cytometry

Flow cytometry analysis of neuronal cells from mock- and LACV-infected COs. Mock- (a–d) and LACV-infected (e–h) COs were non-enzymatically digested into a single cell suspension and analyzed via flow cytometry as described in the “Methods” section. Two representative examples are shown. Live cells were identified and interrogated for expression of activated poly-caspases (y-axis) and LACV expression (x-axis). a, e Active caspase and LACV staining from the whole live cell population. Gating within the whole live cell population for Sox2 (b, f), DCX (c, g) and beta III tubulin-positive cells allowed for examination of active poly-caspase and LACV staining within each neuronal population. Proportions of LACV-infected (i), activated poly-caspase (j), and LACV-infected/activated poly-caspase double-positive (k) neuronal cells within mock (closed circles) or infected (open squares) COs are shown. A two-way ANOVA with a Sidak’s multiple comparisons test was used to determine significance. **p < 0.001, ****p < 0.0001 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31739796), licensed under a CC-BY license. Not internally tested by R&D Systems.