Neuron-specific beta -III Tubulin PerCP-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of beta ‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry. Eight-day differentiated Rat cortical stem cells were stained with Mouse Anti-Neuron-specific beta ‑III Tubulin PerCP‑conjugated Monoclonal Antibody (Catalog # IC1195C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta-III Tubulin
beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium.
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