Detection of beta ‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry.
Eight-day differentiated Rat cortical stem cells were stained with Mouse Anti-Neuron-specific beta ‑III Tubulin PerCP‑conjugated Monoclonal Antibody (Catalog # IC1195C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta-III Tubulin
beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium.
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