Neuron-specific beta -III Tubulin PerCP-conjugated Antibody

IC1195C Datasheet / COA / SDS

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IC1195C

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Detection of beta ‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry.

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Neuron-specific beta -III Tubulin PerCP-conjugated Antibody Summary

Specificity

Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # TuJ-1

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Rat brain-derived microtubules

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry

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Detection of beta ‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry. Eight-day differentiated Rat cortical stem cells were stained with Mouse Anti-Neuron-specific beta ‑III Tubulin PerCP‑conjugated Monoclonal Antibody (Catalog # IC1195C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: beta-III Tubulin

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium.

Entrez Gene IDs

10381 (Human)

Alternate Names

Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen