beta -III Tubulin in Rat Cortical Stem Cells. beta -III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using Neuron-specific beta -III Tubulin Biotinylated Monoclonal Antibody (Catalog # BAM1195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-III Tubulin
Class III beta tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of class III beta -tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium (1, 2).
Caccamo, D. et al. (1989) Lab. Invest. 60:390.
Alexander, J. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4685.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Have you used Neuron-specific beta -III Tubulin Biotinylated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.