Neuron-specific beta-III Tubulin Biotinylated Antibody
Neuron-specific beta-III Tubulin Biotinylated Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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beta -III Tubulin in Rat Cortical Stem Cells. beta -III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using Neuron-specific beta -III Tubulin Biotinylated Monoclonal Antibody (Catalog # BAM1195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-III Tubulin
Class III beta tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of class III beta -tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium (1, 2).
- Caccamo, D. et al. (1989) Lab. Invest. 60:390.
- Alexander, J. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4685.
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Citations for Neuron-specific beta-III Tubulin Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life
Mucosal Immunol, 2016;9(6):1466-1476.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-P -
An NT4/TrkB-dependent increase in innervation links early-life allergen exposure to persistent airway hyperreactivity.
Authors: Aven, Linh, Paez-Cortez, Jesus, Achey, Rebecca, Krishnan, Ramaswam, Ram-Mohan, Sumati, Cruikshank, William, Fine, Alan, Ai, Xingbin
FASEB J, 2014;28(2):897-907.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
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