Neuron-specific beta -III Tubulin Biotinylated Antibody

(3 citations)   
  • Specificity
    Detects mammalian and chicken neuron-specific  beta -III tubulin but not other beta -tubulin isotypes.
  • Source
    Monoclonal Mouse IgG2A Clone # TuJ-1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Rat brain-derived microtubules
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    beta -III Tubulin
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
beta -III Tubulin in Rat Cortical Stem Cells. beta -III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using Neuron-specific beta -III Tubulin Biotinylated Monoclonal Antibody (Catalog # BAM1195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-III Tubulin

Class III beta tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of class III beta -tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium (1, 2).

  • References:
    1. Caccamo, D. et al. (1989) Lab. Invest. 60:390.
    2. Alexander, J. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4685.
  • Alternate Names:
    beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life
    Mucosal Immunol, 2016;9(6):1466-1476.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  2. An NT4/TrkB-dependent increase in innervation links early-life allergen exposure to persistent airway hyperreactivity.
    Authors: Aven, Linh, Paez-Cortez, Jesus, Achey, Rebecca, Krishnan, Ramaswam, Ram-Mohan, Sumati, Cruikshank, William, Fine, Alan, Ai, Xingbin
    FASEB J, 2014;28(2):897-907.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Biotinylated Isotype Control

Ctrl IC003B
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
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Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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