Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # TuJ-1
View all beta-III Tubulin Primary Antibodies »

Product Specifications

Immunogen

Rat brain-derived microtubules

Specificity

Detects mammalian and chicken neuron-specific  beta -III tubulin but not other beta -tubulin isotypes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Neuron-specific beta-III Tubulin Biotinylated Antibody

beta -III Tubulin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

beta -III Tubulin in Rat Cortical Stem Cells.

beta -III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using Neuron-specific beta -III Tubulin Biotinylated Monoclonal Antibody (Catalog # BAM1195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Neuron-specific beta-III Tubulin Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed differentiated rat cortical stem cells

Western Blot

1 µg/mL
Sample: beta -III Tubulin

Reviewed Applications

Read 1 review rated 5 using BAM1195 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-III Tubulin

Class III beta tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of class III beta -tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium (1, 2).

References

  1. Caccamo, D. et al. (1989) Lab. Invest. 60:390.
  2. Alexander, J. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4685.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Entrez Gene IDs

10381 (Human)

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for Neuron-specific beta-III Tubulin Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Neuron-specific beta-III Tubulin Biotinylated Antibody

For research use only

Citations for Neuron-specific beta-III Tubulin Biotinylated Antibody

Customer Reviews for Neuron-specific beta-III Tubulin Biotinylated Antibody (1)

5 out of 5
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  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID: 22031887
    Species: Mouse
    Verified Customer | Posted 01/15/2015

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Protocols

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