Neuron-specific beta -III Tubulin NorthernLights™ NL557-conjugated Antibody

(1 citations)
(1 Review)
  
  • Specificity
    Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # TuJ-1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Rat brain-derived microtubules
  • Formulation
    Supplied as a 10X solution of antibody in PBS containing sodium azide.
  • Label
    NL557
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    1:10 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
beta ‑III Tubulin in Rat Cortical Stem Cells. beta ‑III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using a 1:10 dilution of Neuron-specific beta ‑III Tubulin (Clone TuJ‑1) NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1195R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta-III Tubulin
beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.
  • Alternate Names:
    beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Intranasal delivery of nanomicelle curcumin promotes corneal epithelial wound healing in streptozotocin-induced diabetic mice
    Sci Rep, 2016;6(0):29753.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
Immunohistochemistry Reagents
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NorthernLights Guard Mounting Media

NL996
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Neuron-specific  beta -III Tubulin NorthernLights™ NL557-conjugated Antibody NL1195R
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 ICC/IF Mouse Anonymous 10/27/2017
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Immunocytochemistry/Immunofluorescence Neuron-specific  beta -III Tubulin NorthernLights™ NL557-conjugated Antibody NL1195R
Immunocytochemistry/Immunofluorescence: Neuron-specific beta -III Tubulin NorthernLights™ NL557-conjugated Antibody [NL1195R]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedcorneas
SpeciesMouse

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