Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody

Catalog # Availability Size / Price Qty
NL1195R

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 beta ‑III Tubulin in Rat Cortical Stem Cells.
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Product Details
Citations (8)
FAQs
Reviews (1)

Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody Summary

Specificity
Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.
Source
Monoclonal Mouse IgG2A Clone # TuJ-1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Rat brain-derived microtubules
Formulation
Supplied as a 10X solution of antibody in PBS containing sodium azide.
Label
NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
1:10 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry  beta ‑III Tubulin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

beta ‑III Tubulin in Rat Cortical Stem Cells. beta ‑III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using a 1:10 dilution of Neuron-specific beta ‑III Tubulin (Clone TuJ‑1) NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1195R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry/ Immunofluorescence Detection of Mouse beta-III Tubulin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse beta-III Tubulin by Immunocytochemistry/Immunofluorescence Assessment of the roles of macrophages in corneal development. a Co-staining of limbal blood vessels/corneal nerve fibers and macrophages in mice at postnatal day 7 (P7). The upper image shows the immunostaining of the limbal blood vessels and macrophages with anti-CD31 antibody and anti-CD64 antibody (scale bar, 10 μm). The lower image shows the immunostaining of the corneal nerve fibers and macrophages with anti-beta -III-tubulin antibody and anti-CD64 antibody (scale bar, 10 μm). b The gating strategies of analyzing corneal macrophages with flow cytometry. Corneal macrophages in mice at P7 were classified into C–C chemokine receptor type 2 negative (CCR2−) and positive (CCR2+) populations. The gating strategies were justified by the related isotype controls in Supplementary Fig. 6. c The expression of genes involved in cell division (left image) and neurogenesis and angiogenesis (right image) in the flow-cytometry-sorted corneal CCR2− and CCR2+ macrophages with qPCR (n = 3 independent experiments, ten mice per experiment in each group). d Changes in the corneal development of anti-CSF1R antibody-treated mice (n = 6 mice at each time point in each group). e Alteration of the expression of genes involved in cell division, neurogenesis, and angiogenesis in corneal tissues of anti-CSF1R antibody-treated mice at P28 (n = 4 independent experiments, six mice per experiment in each group). f Changes in the corneal development of BMS CCR2 22-treated mice (n = 6 mice at each time point in each group). The data are presented as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31434991), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: beta-III Tubulin

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Entrez Gene IDs
10381 (Human)
Alternate Names
Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen

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Citations for Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Sensory nerves promote corneal inflammation resolution via CGRP mediated transformation of macrophages to the M2 phenotype through the PI3K/AKT signaling pathway
    Authors: K Yuan, J Zheng, X Shen, Y Wu, Y Han, X Jin, X Huang
    International immunopharmacology, 2021-12-11;102(0):108426.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Shp2-mediated MAPK pathway regulates &deltaNp63 in epithelium to promote corneal innervation and homeostasis
    Authors: Y Okada, Y Zhang, L Zhang, LK Yeh, YC Wang, S Saika, CY Liu
    Lab. Invest., 2019-10-25;100(4):630-642.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Examining the fundamental biology of a novel population of directly reprogrammed human neural precursor cells
    Authors: JE Ahlfors, A Azimi, R El-Ayoubi, A Velumian, I Vonderwald, C Boscher, O Mihai, S Mani, M Samoilova, M Khazaei, MG Fehlings, CM Morshead
    Stem Cell Res Ther, 2019-06-13;10(1):166.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A Novel Murine Model of Radiation Keratopathy
    Authors: DL Harris, T Yamaguchi, P Hamrah
    Invest. Ophthalmol. Vis. Sci., 2018-08-01;59(10):3889-3896.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. The Role of Microglia and Peripheral Monocytes in Retinal Damage Following Corneal Chemical Injury
    Authors: EI Paschalis, F Lei, C Zhou, V Kapoulea, A Thanos, R Dana, D Vavvas, J Chodosh, CH Dohlman
    Am. J. Pathol., 2018-04-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Epothilone B Speeds Corneal Nerve Regrowth and Functional Recovery through Microtubule Stabilization and Increased Nerve Beading
    Authors: H Wang, C Xiao, D Dong, C Lin, Y Xue, J Liu, M Wu, J He, T Fu, H Pan, X Jiao, D Lu, Z Li
    Sci Rep, 2018-02-08;8(1):2647.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Mechanisms of Retinal Damage after Ocular Alkali Burns
    Authors: EI Paschalis, C Zhou, F Lei, N Scott, V Kapoulea, MC Robert, D Vavvas, R Dana, J Chodosh, CH Dohlman
    Am. J. Pathol., 2017-04-13;0(0):.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  8. Intranasal delivery of nanomicelle curcumin promotes corneal epithelial wound healing in streptozotocin-induced diabetic mice
    Sci Rep, 2016-07-11;6(0):29753.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Reviews for Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody

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Neuron-specific beta -III Tubulin NorthernLights™ NL557-conjugated Antibody
By Nan Gao on 10/27/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: corneas Species: Mouse