Home / beta-III Tubulin / Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody

Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody

Catalog #: NL1195R
8 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
NL1195R
beta ‑III Tubulin in Rat Cortical Stem Cells.
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Product Details
Citations (8)
FAQs
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody Summary

Specificity
Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.
Source
Monoclonal Mouse IgG2A Clone # TuJ-1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Rat brain-derived microtubules
Formulation
Supplied as a 10X solution of antibody in PBS containing sodium azide.
Label
NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
1:10 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry beta ‑III Tubulin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

beta ‑III Tubulin in Rat Cortical Stem Cells. beta ‑III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using a 1:10 dilution of Neuron-specific beta ‑III Tubulin (Clone TuJ‑1) NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1195R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: beta-III Tubulin

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Entrez Gene IDs
10381 (Human)
Alternate Names
Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen

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Citations for Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Sensory nerves promote corneal inflammation resolution via CGRP mediated transformation of macrophages to the M2 phenotype through the PI3K/AKT signaling pathway
    Authors: K Yuan, J Zheng, X Shen, Y Wu, Y Han, X Jin, X Huang
    International immunopharmacology, 2022;102(0):108426.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Shp2-mediated MAPK pathway regulates &deltaNp63 in epithelium to promote corneal innervation and homeostasis
    Authors: Y Okada, Y Zhang, L Zhang, LK Yeh, YC Wang, S Saika, CY Liu
    Lab. Invest., 2020;100(4):630-642.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Examining the fundamental biology of a novel population of directly reprogrammed human neural precursor cells
    Authors: JE Ahlfors, A Azimi, R El-Ayoubi, A Velumian, I Vonderwald, C Boscher, O Mihai, S Mani, M Samoilova, M Khazaei, MG Fehlings, CM Morshead
    Stem Cell Res Ther, 2019;10(1):166.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Epothilone B Speeds Corneal Nerve Regrowth and Functional Recovery through Microtubule Stabilization and Increased Nerve Beading
    Authors: H Wang, C Xiao, D Dong, C Lin, Y Xue, J Liu, M Wu, J He, T Fu, H Pan, X Jiao, D Lu, Z Li
    Sci Rep, 2018;8(1):2647.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. A Novel Murine Model of Radiation Keratopathy
    Authors: DL Harris, T Yamaguchi, P Hamrah
    Invest. Ophthalmol. Vis. Sci., 2018;59(10):3889-3896.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. The Role of Microglia and Peripheral Monocytes in Retinal Damage Following Corneal Chemical Injury
    Authors: EI Paschalis, F Lei, C Zhou, V Kapoulea, A Thanos, R Dana, D Vavvas, J Chodosh, CH Dohlman
    Am. J. Pathol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Mechanisms of Retinal Damage after Ocular Alkali Burns
    Authors: EI Paschalis, C Zhou, F Lei, N Scott, V Kapoulea, MC Robert, D Vavvas, R Dana, J Chodosh, CH Dohlman
    Am. J. Pathol., 2017;0(0):.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  8. Intranasal delivery of nanomicelle curcumin promotes corneal epithelial wound healing in streptozotocin-induced diabetic mice
    Sci Rep, 2016;6(0):29753.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Reviews for Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody

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Neuron-specific beta -III Tubulin NorthernLights™ NL557-conjugated Antibody
By Nan Gao on 10/27/2017
Application: ICC/IF Sample Tested: corneas Species: Mouse
Immunocytochemistry/Immunofluorescence Neuron-specific beta -III Tubulin NorthernLights™ NL557-conjugated Antibody NL1195R