Neuron-specific beta -III Tubulin Antibody

  (108 citations)
(7 Reviews)
    
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Product Details
Citations (108)
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  • Specificity
    Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # TuJ-1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Rat brain-derived microtubules
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse beta ‑III Tubulin by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta ‑III Tubulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
beta ‑III Tubulin in Differentiated Human Neural Progenitor Cells. beta ‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours at room temperature. Cells were stained (green) and counterstained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
beta ‑III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes. beta ‑III Tubulin was detected in rat cortical neurons using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for
beta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the NorthernLights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
beta ‑III Tubulin and Nestin in Rat Cortical Stem Cells.
beta ‑III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195) and 10 µg/mL Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Rat beta ‑III Tubulin by Simple WesternTM. Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta ‑III Tubulin at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-III Tubulin
beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.
  • Entrez Gene IDs:
    10381 (Human)
  • Alternate Names:
    Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

108 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Regulation of neuritogenesis in hippocampal neurons using stiffness of extracellular microenvironment
    Authors: A Tanaka, Y Fujii, N Kasai, T Okajima, H Nakashima
    PLoS ONE, 2018;13(2):e0191928.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  2. Generation of multidrug resistant human tissues by overexpression of the ABCG2 multidrug transporter in embryonic stem cells
    Authors: Z Erdei, A Schamberge, G Török, K Szebényi, G Várady, TI Orbán, L Homolya, B Sarkadi, Á Apáti
    PLoS ONE, 2018;13(4):e0194925.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Nitrosative damage during retrovirus infection-induced neuropathic pain
    Authors: P Chauhan, WS Sheng, S Hu, S Prasad, JR Lokensgard
    J Neuroinflammation, 2018;15(1):66.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction
    Authors: JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman
    Mol Ther Methods Clin Dev, 2018;9(0):172-180.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement
    Authors: E Emmerson, AJ May, L Berthoin, N Cruz-Pache, S Nathan, AJ Mattingly, JL Chang, WR Ryan, AD Tward, SM Knox
    EMBO Mol Med, 2018;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  7. Vulnerability to oxygen-glucose deprivation of primary neurons derived from Tg2576 Alzheimer mice: role of intraneuronal Abeta accumulation and astrocytes
    Authors: VA Baldassarr, A Marchesini, L Giardino, L Calzà
    Dis Model Mech, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  8. An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation
    Authors: J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski
    Cell Rep, 2017;19(2):281-294.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Transfer of pathogenic and nonpathogenic cytosolic proteins between spinal cord motor neurons in vivo in chimeric mice
    Authors: EV Thomas, WA Fenton, J McGrath, AL Horwich
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  10. PEGylated insulin-like growth factor-I affords protection and facilitates recovery of lost functions post-focal ischemia
    Authors: K Parker, A Berretta, S Saenger, M Sivaramakr, SA Shirley, F Metzger, AN Clarkson
    Sci Rep, 2017;7(1):241.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  11. Embryonic Stem Cell-Derived Neurons Grown on Multi-Electrode Arrays as a Novel In vitro Bioassay for the Detection of Clostridium botulinum Neurotoxins
    Authors: SP Jenkinson, D Grandgirar, M Heidemann, A Tscherter, MA Avondet, SL Leib
    Front Pharmacol, 2017;8(0):73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  12. Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development
    Authors: K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  13. NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis
    Authors: SC Hrstka, X Li, TJ Nelson,
    Stem Cells, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Zeb2 is a negative regulator of midbrain dopaminergic axon growth and target innervation
    Authors: SV Hegarty, SL Wyatt, L Howard, E Stappers, D Huylebroec, AM Sullivan, GW O'Keeffe
    Sci Rep, 2017;7(1):8568.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  15. Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions
    Authors: J Kon¡?ov , J Oltov , A Corlett, J Kopyci?ska, M Kol ?, P Bart?n?k, M Z¡kov 
    Sci Rep, 2017;7(0):41597.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  16. Poly(ADP-ribose) polymerase inhibitors activate the p53 signaling pathway in neural stem/progenitor cells
    Authors: A Okuda, S Kurokawa, M Takehashi, A Maeda, K Fukuda, Y Kubo, H Nogusa, T Takatani-N, S Okuda, K Ueda, S Tanaka
    BMC Neurosci, 2017;18(1):14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  17. A novel role for the DNA repair gene Rad51 in Netrin-1 signalling
    Authors: KA Glendining, D Markie, RJ Gardner, EA Franz, SP Robertson, CL Jasoni
    Sci Rep, 2017;7(0):39823.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  18. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017;19(1):20-35.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  19. Enforcement of developmental lineage specificity by transcription factor Oct1
    Authors: Z Shen, J Kang, A Shakya, M Tabaka, EA Jarboe, A Regev, D Tantin
    Elife, 2017;6(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  20. HDAC3 inhibition ameliorates spinal cord injury by immunomodulation
    Authors: T Kuboyama, S Wahane, Y Huang, X Zhou, JK Wong, A Koemeter-C, M Martini, RH Friedel, H Zou
    Sci Rep, 2017;7(1):8641.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  21. Molecular and Functional Properties of Regional Astrocytes in the Adult Brain
    Authors: L Morel, MSR Chiang, H Higashimor, T Shoneye, LK Iyer, J Yelick, A Tai, Y Yang
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  22. Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging
    Authors: T Ishii, E Kawakami, K Endo, H Misawa, K Watabe
    PLoS ONE, 2017;12(6):e0179375.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  23. PARP activity and inhibition in fetal and adult oligodendrocyte precursor cells: Effect on cell survival and differentiation
    Authors: VA Baldassarr, A Marchesini, L Giardino, L Calzà
    Stem Cell Res, 2017;22(0):54-60.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  24. Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport
    Authors: S Toba, M Jin, M Yamada, K Kumamoto, S Matsumoto, T Yasunaga, Y Fukunaga, A Miyazawa, S Fujita, K Itoh, S Fushiki, H Kojima, H Wanibuchi, Y Arai, T Nagai, S Hirotsune
    Sci Rep, 2017;7(1):16386.
    Species: Rat
    Sample Type: Tissue Homogenate
    Application: IP
  25. FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1
    Authors: K Hirano, M Namihira
    FEBS Open Bio, 2017;7(12):1932-1942.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  26. Preclinical analysis of MTOR complex 1/2 inhibition in diffuse intrinsic pontine glioma
    Authors: PC Flannery, JA DeSisto, V Amani, S Venkataram, RT Lemma, EW Prince, A Donson, EE Moroze, L Hoffman, JMM Levy, N Foreman, R Vibhakar, AL Green
    Oncol. Rep., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  27. Repeated removal of developing limb buds permanently reduces appendage size in the highly-regenerative axolotl
    Authors: DM Bryant, K Sousounis, JE Farkas, S Bryant, N Thao, AR Guzikowski, JR Monaghan, M Levin, JL Whited
    Dev. Biol, 2017;0(0):.
    Species: Axolotl - Ambystoma mexicanum
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  28. Neuronal cells derived from human induced pluripotent stem cells as a functional tool of melanocortin system
    Authors: N Yamada-Got, Y Ochi, G Katsuura, Y Yamashita, K Ebihara, M Noguchi, J Fujikura, D Taura, M Sone, K Hosoda, PE Gottschall, K Nakao
    Neuropeptides, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  29. An Immortalized Human Dorsal Root Ganglia Cell Line Provides a Novel Context to Study Herpes Simplex Virus Type-1 Latency and Reactivation
    Authors: NM Thellman, C Botting, Z Madaj, SJ Triezenber
    J. Virol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  30. Selective Deletion of Astroglial FMRP Dysregulates Glutamate Transporter GLT1 and Contributes to Fragile X Syndrome Phenotypes In Vivo
    J Neurosci, 2016;36(27):7079-94.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  31. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9
    Stem Cell Res, 2016;17(2):430-432.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  32. The Severity of Infection Determines the Localization of Damage and Extent of Sensorineural Hearing Loss in Experimental Pneumococcal Meningitis
    J Neurosci, 2016;36(29):7740-9.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  33. FGF8 and SHH substitute for anterior-posterior tissue interactions to induce limb regeneration
    Nature, 2016;533(7603):407-10.
    Species: Axolotl
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  34. IL-20 promotes epithelial healing of the injured mouse cornea
    Authors: Alan R Burns
    Exp. Eye Res., 2016;154(0):22-29.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  35. Spinal cord injury induces astroglial conversion towards neuronal lineage
    Mol Neurodegener, 2016;11(1):68.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  36. Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9
    Stem Cell Res, 2016;17(2):441-443.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Fresh
  37. VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination
    Nat Commun, 2016;7(0):13478.
    Species: Rat
    Sample Type: Whole Cells
    Application: IHC Not Specified
  38. Cytosine-5 RNA Methylation Regulates Neural Stem Cell Differentiation and�Motility
    Stem Cell Reports, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  39. Retinoic acid-mediated HRas stabilization induces neuronal differentiation of neural stem cells during brain development
    J Cell Sci, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  40. A system to study mechanisms of neuromuscular junction development and maintenance
    Development, 2016;0(0):.
    Species: Rat
    Sample Type: Whole Cells
    Application: IHC Not Specified
  41. IL-20 promotes epithelial healing of the injured mouse cornea
    Exp. Eye Res., 2016;154(0):22-29.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  42. Assembly and interrogation of Alzheimer's disease genetic networks reveal novel regulators of progression.
    Authors: Aubry S, Shin W, Crary J, Lefort R, Qureshi Y, Lefebvre C, Califano A, Shelanski M
    PLoS ONE, 2015;10(3):e0120352.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  43. Angiogenesis in the developing spinal cord: blood vessel exclusion from neural progenitor region is mediated by VEGF and its antagonists.
    Authors: Takahashi, Teruaki, Takase, Yuta, Yoshino, Takashi, Saito, Daisuke, Tadokoro, Ryosuke, Takahashi, Yoshiko
    PLoS ONE, 2015;10(1):e0116119.
    Species: Avian - Quail
    Sample Type: Whole Tissue
    Application: IHC Frozen
  44. Attenuation of Progressive Hearing Loss in DBA/2J Mice by Reagents that Affect Epigenetic Modifications Is Associated with Up-Regulation of the Zinc Importer Zip4.
    Authors: Mutai H, Miya F, Fujii M, Tsunoda T, Matsunaga T
    PLoS ONE, 2015;10(4):e0124301.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  45. Signalling Through Retinoic Acid Receptors is Required for Reprogramming of Both Mouse Embryonic Fibroblast Cells and Epiblast Stem Cells to Induced Pluripotent Stem Cells.
    Authors: Yang J, Wang W, Ooi J, Campos L, Lu L, Liu P
    Stem Cells, 2015;33(5):1390-404.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  46. Developmental regulation of trigeminal TRPA1 by the cornea.
    Authors: Canner J, Linsenmayer T, Kubilus J
    Invest Ophthalmol Vis Sci, 2015;56(1):29-36.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  47. A simple and robust method for establishing homogeneous mouse epiblast stem cell lines by wnt inhibition.
    Authors: Sugimoto M, Kondo M, Koga Y, Shiura H, Ikeda R, Hirose M, Ogura A, Murakami A, Yoshiki A, Chuva de Sousa Lopes S, Abe K
    Stem Cell Reports, 2015;4(4):744-57.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  48. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
    Authors: Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
    J Neurosci, 2015;35(6):2452-64.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  49. An Engineered N-Cadherin Substrate for Differentiation, Survival, and Selection of Pluripotent Stem Cell-Derived Neural Progenitors.
    Authors: Haque A, Adnan N, Motazedian A, Akter F, Hossain S, Kutsuzawa K, Nag K, Kobatake E, Akaike T
    PLoS ONE, 2015;10(8):e0135170.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Fresh
  50. Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells.
    Authors: Hagiwara, Kunie, Obayashi, Takeshi, Sakayori, Nobuyuki, Yamanishi, Emiko, Hayashi, Ryuhei, Osumi, Noriko, Nakazawa, Toru, Nishida, Kohji
    PLoS ONE, 2014;9(1):e84072.
  51. Growth arrest and forced differentiation of human primary glioblastoma multiforme by a novel small molecule.
    Authors: Kang, Tae-Wook, Choi, Soon Won, Yang, Se-Ran, Shin, Tae-Hoon, Kim, Hyung-Si, Yu, Kyung-Ro, Hong, In-Sun, Ro, Seonggu, Cho, Joong My, Kang, Kyung-Su
    Sci Rep, 2014;4(0):5546.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  52. Intrinsically active and pacemaker neurons in pluripotent stem cell-derived neuronal populations.
    Authors: Illes S, Jakab M, Beyer F, Gelfert R, Couillard-Despres S, Schnitzler A, Ritter M, Aigner L
    Stem Cell Reports, 2014;2(3):323-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC
  53. Molecular evidence for OCT4-induced plasticity in adult human fibroblasts required for direct cell fate conversion to lineage specific progenitors.
    Authors: Mitchell R, Szabo E, Shapovalova Z, Aslostovar L, Makondo K, Bhatia M
    Stem Cells, 2014;32(8):2178-87.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  54. Netrin-1 controls sympathetic arterial innervation.
    Authors: Brunet I, Gordon E, Han J, Cristofaro B, Broqueres-You D, Liu C, Bouvree K, Zhang J, Del Toro R, Mathivet T, Larrivee B, Jagu J, Pibouin-Fragner L, Pardanaud L, Machado M, Kennedy T, Zhuang Z, Simons M, Levy B, Tessier-Lavigne M, Grenz A, Eltzschig H, Eichmann A
    J Clin Invest, 2014;124(7):3230-40.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  55. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression.
    Authors: Ferrarese R, Harsh G, Yadav A, Bug E, Maticzka D, Reichardt W, Dombrowski S, Miller T, Masilamani A, Dai F, Kim H, Hadler M, Scholtens D, Yu I, Beck J, Srinivasasainagendra V, Costa F, Baxan N, Pfeifer D, von Elverfeldt D, Backofen R, Weyerbrock A, Duarte C, He X, Prinz M, Chandler J, Vogel H, Chakravarti A, Rich J, Carro M, Bredel M
    J Clin Invest, 2014;124(7):2861-76.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  56. Diet-induced obesity and low testosterone increase neuroinflammation and impair neural function.
    Authors: Jayaraman, Anusha, Lent-Schochet, Daniella, Pike, Christia
    J Neuroinflammation, 2014;11(0):162.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  57. Maternal obesity and IL-6 lead to aberrant developmental gene expression and deregulated neurite growth in the fetal arcuate nucleus.
    Authors: Sanders, Tessa R, Kim, Dong Won, Glendining, Kelly A, Jasoni, Christin
    Endocrinology, 2014;155(7):2566-77.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  58. CaMKK-CaMK1a, a new post-traumatic signalling pathway induced in mouse somatosensory neurons.
    Authors: Elziere L, Sar C, Venteo S, Bourane S, Puech S, Sonrier C, Boukhadaoui H, Fichard A, Pattyn A, Valmier J, Carroll P, Mechaly I
    PLoS ONE, 2014;9(5):e97736.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  59. Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions.
    Authors: Durruthy-Durruthy, Jens, Briggs, Sharon F, Awe, Jason, Ramathal, Cyril Y, Karumbayaram, Saravana, Lee, Patrick, Heidmann, Julia D, Clark, Amander, Karakikes, Ioannis, Loh, Kyle M, Wu, Joseph C, Hoffman, Andrew R, Byrne, James, Reijo Pera, Renee A, Sebastiano, Vittorio
    PLoS ONE, 2014;9(4):e94231.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  60. Nogo receptor homolog NgR2 expressed in sensory DRG neurons controls epidermal innervation by interaction with Versican.
    Authors: Baumer B, Kurz A, Borrie S, Sickinger S, Dours-Zimmermann M, Zimmermann D, Bandtlow C
    J Neurosci, 2014;34(5):1633-46.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  61. Stage-dependent requirement of neuroretinal Pax6 for lens and retina development.
    Authors: Klimova, Lucie, Kozmik, Zbynek
    Development, 2014;141(6):1292-302.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  62. ADAM metalloproteases promote a developmental switch in responsiveness to the axonal repellant Sema3A.
    Authors: Romi E, Gokhman I, Wong E, Antonovsky N, Ludwig A, Sagi I, Saftig P, Tessier-Lavigne M, Yaron A
    Nat Commun, 2014;5(0):4058.
  63. Influence of biological matrix and artificial electrospun scaffolds on proliferation, differentiation and trophic factor synthesis of rat embryonic stem cells.
    Authors: Alessandri M, Lizzo G, Gualandi C, Mangano C, Giuliani A, Focarete M, Calza L
    Matrix Biol, 2014;33(0):68-76.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  64. Absence of lipofuscin in motor neurons of SOD1-linked ALS mice.
    Authors: Bandyopadhyay U, Nagy M, Fenton W, Horwich A
    Proc Natl Acad Sci U S A, 2014;111(30):11055-60.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  65. Senescence impairs direct conversion of human somatic cells to neurons.
    Authors: Sun, Chong-Ku, Zhou, Di, Zhang, Zhen, He, Liming, Zhang, Fan, Wang, Xiaowei, Yuan, Jie, Chen, Qianming, Wu, Ling-Gan, Yang, Qin
    Nat Commun, 2014;5(0):4112.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Not Specified
  66. Cytokine secretion from brain macrophages infected with human immunodeficiency virus in vitro and treated with raltegravir.
    Authors: Tatro E, Soontornniyomkij B, Letendre S, Achim C
    BMC Infect Dis, 2014;14(0):386.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Not Specified
  67. Axolotls with an under- or oversupply of neural crest can regulate the sizes of their dorsal root ganglia to normal levels.
    Authors: Zarzosa A, Grassme K, Tanaka E, Taniguchi Y, Bramke S, Kurth T, Epperlein H
    Dev Biol, 2014;394(1):65-82.
    Species: Axolotl
    Sample Type: Whole Cells
    Application: IHC OCT-embedded
  68. Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma.
    Authors: Hu S, Rotschafer J, Lokensgard J, Cheeran M
    PLoS ONE, 2014;9(8):e105219.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  69. ProNGF promotes neurite growth from a subset of NGF-dependent neurons by a p75NTR-dependent mechanism.
    Authors: Howard, Laura, Wyatt, Sean, Nagappan, Guhan, Davies, Alun M
    Development, 2013;140(10):2108-17.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  70. Genetic deletion of afadin causes hydrocephalus by destruction of adherens junctions in radial glial and ependymal cells in the midbrain.
    Authors: Yamamoto H, Maruo T, Majima T, Ishizaki H, Tanaka-Okamoto M, Miyoshi J, Mandai K, Takai Y
    PLoS ONE, 2013;8(11):e80356.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  71. Lamin A/C haploinsufficiency modulates the differentiation potential of mouse embryonic stem cells.
    Authors: Sehgal P, Chaturvedi P, Kumaran R, Kumar S, Parnaik V
    PLoS ONE, 2013;8(2):e57891.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IHC
  72. Parasympathetic stimulation improves epithelial organ regeneration.
    Authors: Knox, Sarah M, Lombaert, Isabelle, Haddox, Candace, Abrams, Shaun R, Cotrim, Ana, Wilson, Adrian J, Hoffman, Matthew
    Nat Commun, 2013;4(0):1494.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  73. ERK2 suppresses self-renewal capacity of embryonic stem cells, but is not required for multi-lineage commitment.
    Authors: Hamilton W, Kaji K, Kunath T
    PLoS ONE, 2013;8(4):e60907.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  74. Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.
    Authors: Ye S, Tan L, Yang R, Fang B, Qu S, Schulze EN, Song H, Ying Q, Li P
    PLoS ONE, 2012;7(4):e35892.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  75. In vivo generation of neural tumors from neoplastic pluripotent stem cells models early human pediatric brain tumor formation.
    Authors: Werbowetski-Ogilvie TE, Morrison LC, Fiebig-Comyn A, Bhatia M
    Stem Cells, 2012;30(3):392-404.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  76. Communication between corneal epithelial cells and trigeminal neurons is facilitated by purinergic (P2) and glutamatergic receptors.
    Authors: Oswald, Duane J, Lee, Albert, Trinidad, Monique, Chi, Cheryl, Ren, Ruiyi, Rich, Celeste, Trinkaus-Randall, Vickery
    PLoS ONE, 2012;7(9):e44574.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  77. IL-17 and VEGF are necessary for efficient corneal nerve regeneration.
    Authors: Li Z, Burns AR, Han L, Rumbaut RE, Smith CW
    Am. J. Pathol., 2011;178(3):1106-16.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  78. Metastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation.
    Authors: Yamano N, Kimura T, Watanabe-Kushima S, Shinohara T, Nakano T
    Biochem. Biophys. Res. Commun., 2010;392(3):311-6.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  79. Developmental guidance of embryonic corneal innervation: roles of Semaphorin3A and Slit2.
    Authors: Kubilus JK, Linsenmayer TF,
    Dev. Biol., 2010;344(1):172-84.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC Frozen
  80. Presence of pluripotent CD133+ cells correlates with malignancy of gliomas.
    Authors: Thon N, Damianoff K, Hegermann J, Grau S, Krebs B, Schnell O, Tonn JC, Goldbrunner R
    Mol. Cell. Neurosci., 2010;43(1):51-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  81. Synovial sarcoma is a stem cell malignancy.
    Authors: Naka N, Takenaka S, Araki N
    Stem Cells, 2010;28(7):1119-31.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  82. Pig epiblast stem cells depend on activin/nodal signaling for pluripotency and self-renewal.
    Authors: Alberio R, Croxall N, Allegrucci C
    Stem Cells Dev., 2010;19(10):1627-36.
    Species: Porcine
    Sample Type: Whole Cells
    Application: ICC
  83. Astrocyte-derived interleukin-6 promotes specific neuronal differentiation of neural progenitor cells from adult hippocampus.
    Authors: Oh J, McCloskey MA, Blong CC
    J. Neurosci. Res., 2010;88(13):2798-809.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  84. A fusion protein N-cadherin-Fc as an artificial extracellular matrix surface for maintenance of stem cell features.
    Authors: Yue XS, Murakami Y, Tamai T, Nagaoka M, Cho CS, Ito Y, Akaike T
    Biomaterials, 2010;31(20):5287-96.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  85. Developmental corneal innervation: interactions between nerves and specialized apical corneal epithelial cells.
    Authors: Kubilus JK, Linsenmayer TF
    Invest. Ophthalmol. Vis. Sci., 2010;51(2):782-9.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC Frozen
  86. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010;7(12):989-94.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  87. Cells keep a memory of their tissue origin during axolotl limb regeneration.
    Authors: Kragl M, Knapp D, Nacu E, Khattak S, Maden M, Epperlein HH, Tanaka EM
    Nature, 2009;460(7251):60-5.
    Species: Amphibian
    Sample Type: Whole Tissue
    Application: IHC Frozen
  88. Tumor microvasculature supports proliferation and expansion of glioma-propagating cells.
    Authors: Borovski T, Verhoeff JJ, ten Cate R, Cameron K, de Vries NA, van Tellingen O, Richel DJ, van Furth WR, Medema JP, Sprick MR
    Int. J. Cancer, 2009;125(5):1222-30.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  89. Continuous exposure to 900MHz GSM-modulated EMF alters morphological maturation of neural cells.
    Authors: Del Vecchio G, Giuliani A, Fernandez M, Mesirca P, Bersani F, Pinto R, Ardoino L, Lovisolo GA, Giardino L, Calza L
    Neurosci. Lett., 2009;455(3):173-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  90. Docosahexaenoic acid promotes neuronal differentiation by regulating basic helix-loop-helix transcription factors and cell cycle in neural stem cells.
    Authors: Katakura M, Hashimoto M, Shahdat HM, Gamoh S, Okui T, Matsuzaki K, Shido O
    Neuroscience, 2009;160(3):651-60.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  91. Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin.
    Authors: Danielyan L, Schafer R, Schulz A, Ladewig T, Lourhmati A, Buadze M, Schmitt AL, Verleysdonk S, Kabisch D, Koeppen K, Siegel G, Proksch B, Kluba T, Eckert A, Kohle C, Schoneberg T, Northoff H, Schwab M, Gleiter CH
    Cell Death Differ., 2009;16(12):1599-614.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  92. Pluripotency associated genes are reactivated by chromatin-modifying agents in neurosphere cells.
    Authors: Ruau D, Ensenat-Waser R, Dinger TC, Vallabhapurapu DS, Rolletschek A, Hacker C, Hieronymus T, Wobus AM, Muller AM, Zenke M
    Stem Cells, 2008;26(4):920-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  93. Autophagy induction and autophagosome clearance in neurons: relationship to autophagic pathology in Alzheimer's disease.
    Authors: Boland B, Kumar A, Lee S, Platt FM, Wegiel J, Yu WH, Nixon RA
    J. Neurosci., 2008;28(27):6926-37.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  94. The free-radical scavenger edaravone restores the differentiation of human neural precursor cells after radiation-induced oxidative stress.
    Authors: Ishii J, Natsume A, Wakabayashi T, Takeuchi H, Hasegawa H, Kim SU, Yoshida J
    Neurosci. Lett., 2007;423(3):225-30.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  95. Phosphorylated FTY720 promotes astrocyte migration through sphingosine-1-phosphate receptors.
    Authors: Mullershausen F, Craveiro LM, Shin Y, Cortes-Cros M, Bassilana F, Osinde M, Wishart WL, Guerini D, Thallmair M, Schwab ME, Sivasankaran R, Seuwen K, Dev KK
    J. Neurochem., 2007;102(4):1151-61.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  96. Reticulon RTN2B regulates trafficking and function of neuronal glutamate transporter EAAC1.
    Authors: Liu Y, Vidensky S, Ruggiero AM, Maier S, Sitte HH, Rothstein JD
    J. Biol. Chem., 2007;283(10):6561-71.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  97. SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
    Authors: Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP
    Development, 2007;134(18):3327-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  98. Triggering neural differentiation of ES cells by subtype switching of importin-alpha.
    Authors: Yasuhara N, Shibazaki N, Nagai M, Kamikawa Y, Oe S, Asally M, Kamachi Y, Kondoh H, Yoneda Y
    Nat. Cell Biol., 2007;9(1):72-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  99. Embryonic stem cell-derived neuron models of Parkinson's disease exhibit delayed neuronal death.
    Authors: Yamashita H, Nakamura T, Takahashi T, Nagano Y, Hiji M, Hirabayashi T, Amano T, Yagi T, Sakai N, Kohriyama T, Matsumoto M
    J. Neurochem., 2006;98(1):45-56.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  100. Neural differentiation of embryonic stem cells induced by conditioned medium from neural stem cell.
    Authors: Zhang JQ, Yu XB, Ma BF, Yu WH, Zhang AX, Huang G, Mao FF, Zhang XM, Wang ZC, Li SN, Lahn BT, Xiang AP
    Neuroreport, 2006;17(10):981-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  101. Docosahexaenoic acid promotes neurogenesis in vitro and in vivo.
    Authors: Kawakita E, Hashimoto M, Shido O
    Neuroscience, 2006;139(3):991-7.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  102. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006;580(25):5869-74.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  103. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm.
    Authors: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM
    Development, 2006;133(19):3787-96.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  104. Isolation of an adult blood-derived progenitor cell population capable of differentiation into angiogenic, myocardial and neural lineages.
    Authors: Porat Y, Porozov S, Belkin D, Shimoni D, Fisher Y, Belleli A, Czeiger D, Silverman WF, Belkin M, Battler A, Fulga V, Savion N
    Br. J. Haematol., 2006;135(5):703-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  105. Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea.
    Authors: Shimmura S, Nagoshi N, Matsuzaki Y, Okano H, Tsubota K
    Stem Cells, 2006;24(12):2714-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  106. 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase form a supramolecular complex in human neutrophils that undergoes retrograde trafficking during pregnancy.
    Authors: Kindzelskii AL, Ueki T, Michibata H, Chaiworapongsa T, Romero R, Petty HR
    J. Immunol., 2004;172(10):6373-81.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  107. Predominant expression of GluR2 among the AMPA receptor subunits in neuronal progenitor cells of the rat hippocampus.
    Authors: Hagimura N, Tsuzuki K, Iino M, Takatsuru Y, Yoshida Y, Kishi S, Ozawa S
    Brain Res. Dev. Brain Res., 2004;152(2):213-23.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  108. High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
    Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
    Brain Res. Dev. Brain Res., 2004;152(2):159-69.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Average Rating: 4.6 (Based on 7 reviews)

Have you used Neuron-specific beta -III Tubulin Antibody?

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Read what people are saying who have used Neuron-specific beta -III Tubulin Antibody.

We have 7 reviews tested in 3 species: Human, Mouse, Rat.
We have 7 reviews tested in 3 applications: Immunohistochemistry, Western Blot, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 IHC Rat Anonymous 04/24/2018
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Summary

Application Immunohistochemistry
Sample Tested Cortical stem cells
Species Rat
  Excellent
 IHC Mouse Anonymous 04/13/2018
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Summary

Application Immunohistochemistry
Sample Tested Serum
Species Mouse
Western Blot Neuron-specific  beta -III Tubulin Antibody MAB1195 Array MAB1195
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Very Good
 WB Mouse Anonymous 01/25/2018
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Western Blot Neuron-specific  beta -III Tubulin Antibody MAB1195
Western Blot: Neuron-specific beta -III Tubulin Antibody [MAB1195].

Summary

Application Western Blot
Sample Tested IPS2 induced pluripotent stem cells
Species Mouse
Western Blot Neuron-specific  beta -III Tubulin Antibody MAB1195 Array MAB1195
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Very Good
 WB Mouse Anonymous 01/25/2018
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Western Blot Neuron-specific  beta -III Tubulin Antibody MAB1195
Western Blot: Neuron-specific beta -III Tubulin Antibody [MAB1195].

Summary

Application Western Blot
Sample Tested IPS2 induced pluripotent stem cells
Species Mouse
  Excellent
 ICC/IF Human Anonymous 05/10/2017
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Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Human fibroblast and human neural stem cells
Species Human
  Excellent
 WB Mouse Anonymous 04/23/2017
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Summary

Application Western Blot
Sample Tested Cortical neurons
Species Mouse
Immunocytochemistry/Immunofluorescence Neuron-specific  beta -III Tubulin Antibody MAB1195 Array MAB1195
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Very Good
 ICC/IF Human Anonymous 04/13/2017
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Immunocytochemistry/Immunofluorescence Neuron-specific  beta -III Tubulin Antibody MAB1195
Immunocytochemistry/Immunofluorescence: Neuron-specific beta -III Tubulin Antibody [MAB1195].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Neural progenitor cells
Species Human

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