Human Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-91266

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid, and seminal plasma (100 uL (1:100 prediluted))

Sensitivity

1.98 pg/ml

Assay Range

0.16 - 10.0 ng/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human PMN Elastase positive sample. There was no cross reactivity or interference detected.

Description

The human PMN elastase ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human PMN elastase

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.80%

Inter-Assay Precision (Precision between assays) CV% 5.60%

Recovery for Human Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 96% to 110% with an overall mean recovery of 104%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

ELISA: Human Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric) [NBP1-91266]

ELISA: Human Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric) [NBP1-91266]

ELISA: Neutrophil Elastase/ELA2 ELISA Kit [NBP1-91266] - Standard curve.

Kit Contents for Human Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human PMN Elastase
  • Control high, lyophilized
  • Control low, lyophilized
  • HRP-Conjugate anti-alpha1-PI polyclonal antibody, ready to use
  • Human PMN Elastase Standard lyophilized, 10 ng/mL upon reconstitution
  • Sample Diluent
  • Stop Solution (2M Hydrochloric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate (10x)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Neutrophil Elastase/ELA2

The human organism reacts with an inflammatory response to attacks of invading pathogens (microorganisms and viruses) or damaged tissue (after accidents or surgery). Polymorphonuclear (PMN) granulocytes play an important role as primary defence cells in this inflammatory reaction. Different bloodstream mediators (cytokines, leukotrienes, complement factors, bacterial endotoxins, clotting and fibrinolysis factors) attract and stimulate these cells to phagocytize and destroy not naturally occurring agents. PMN granulocytes use proteinases to digest these agents and tissue debris. One of these proteinases is PMN elastase which is localised in the azurophilic granules of the polymorphonuclear granulocytes. During phagocytosis of foreign substances these enzymes are also partially excreted into the extracellular surrounding, where the activity of PMN elastase is regulated by inhibitors (esp. the alpha 1-proteinase inhibitor, alpha 1- PI). An overwhelming release of PMN elastase, however, can exceed the inhibitory potential of the alpha 1-proteinase inhibitor. Thus, enzymatically active PMN elastase, together with simultaneously produced oxidants (O2-radicals, H2O, OH-radicals), can cause local tissue injury. Due to the bloodstream and lymphatic system, however, alpha 1-PI is delivered subsequently and eventually able to form a complex with all excreted elastase. Therefore, the concentration of the PMN elastase/alpha 1- PI complex correlates with the released PMN elastase and can be used as a measure for the activity of granulocytes during an inflammatory response. Primarily, determinations of PMN elastase find its application in observation of the course of trauma, shock and sepsis. Further indications are the areas of hemodialysis, infections by obstetrics, joint diseases, effusions of sport injuries, intestinal affection, pancreatitis, cystic fibrosis and male adnex affections.

Alternate Names

ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin

Gene Symbol

ELANE

Additional Neutrophil Elastase/ELA2 Products

Product Documents for Human Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

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FAQs for Human Neutrophil Elastase/ELA2 ELISA Kit (Colorimetric)

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  • Q: Although MSDS of cat # NBP1-91266 could be opened, each concentration of regulated materials (Sodium azide and Acid) were not specified. 1) Sodium azide - Please let us know in which following vial(s) sodium azide is contained. We also would like to know the concentration. 2) Acid - The composition of Stop Solution on your website (1M Phosphoric acid) is different from the one in MSDS (Hydrochloric acid). Would you tell us which one is the correct information? If it is Hydrochloric acid, please let us know the concentration.

    A: 1. The HRP-Conjugate antibody contains less than 0.1% sodium azide. 2. The stop solution is 2M hydrochloric acid. This was a mistake on our website and has since been corrected.

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